Literature summary for 3.4.24.87 extracted from

Gogia, S.; Lo, C.Y.; Neelamegham, S.
Detection of plasma protease activity using microsphere-cytometry assays with E. coli derived substrates: VWF proteolysis by ADAMTS13 (2015), PLoS ONE, 10, e0126556.

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Application

Application	Comment	Organism
analysis	development of a 77-amino acid flow cytometry substrate to measure ADAMTS13 activity. Substrate is suitable for microsphere-cytometer based assays in the milieu of human blood plasma. It contains the partial A2-domain of von Willebrand factor (VWF amino acids 1594-1670) that is mutated to include a single primary amine at the N-terminus and free cysteines at the C-terminus. Substrate is bound to streptavidin-bearing microspheres with varyable polyethylene glycol spacer lengths. Recombinant ADAMTS13 activity can be quantified using substrates with all varyable polyethylene glycol repeat-lengths, but only a construct with the longer 77 PEG-unit can quantify proteolysis in blood plasma. Plasma ADAMTS13 down to 5% of normal levels can be detected within 30 min. Enzyme catalytic activity is tuned by varying buffer calcium, with lower divalent ion concentrations enhancing cleavage	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
Ca2+	enzyme catalytic activity in blood plasma is tuned by varying buffer calcium, with lower divalent ion concentrations enhancing cleavage	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q76LX8	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
blood plasma	-	Homo sapiens	-

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Release 2023.1 (January 2023)