KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
S80-glycosylated (EDANS)-EPLAQAVRSSSK-(DABCYL) | extracellular domain, 25°C, pH not specified in the publication | Homo sapiens | |
0.0032 | - |
S80-glycosylated (EDANS)-EPLAQAVRSSSK-(DABCYL) | extracellular domain, presence of L-galactose, 25°C, pH not specified in the publication | Homo sapiens | |
0.0034 | - |
(EDANS)-EPLAQAVRSSSK-(DABCYL) | catalytic domain, presence of L-galactose, 25°C, pH not specified in the publication | Homo sapiens | |
0.004 | - |
(EDANS)-EPLAQAVRSSSK-(DABCYL) | catalytic domain, 25°C, pH not specified in the publication | Homo sapiens | |
0.004 | - |
(EDANS)-EPLAQAVRSSSK-(DABCYL) | extracellular domain, presence of L-galactose, 25°C, pH not specified in the publication | Homo sapiens | |
0.0069 | - |
S80-glycosylated (EDANS)-EPLAQAVRSSSK-(DABCYL) | catalytic domain, 25°C, pH not specified in the publication | Homo sapiens | |
0.0072 | - |
(EDANS)-ELAQAVRSSSRK-(DABCYL) | extracellular domain, 25°C, pH not specified in the publication | Homo sapiens | |
0.0076 | - |
S80-glycosylated (EDANS)-ELAQAVRSSSRK-(DABCYL) | extracellular domain, 25°C, pH not specified in the publication | Homo sapiens | |
0.01 | - |
S80-glycosylated (EDANS)-EPLAQAVRSSSK-(DABCYL) | catalytic domain, presence of L-galactose, 25°C, pH not specified in the publication | Homo sapiens | |
0.012 | - |
(EDANS)-EPLAQAVRSSSK-(DABCYL) | extracellular domain, 25°C, pH not specified in the publication | Homo sapiens | |
0.016 | - |
(EDANS)-ELAQAVRSSSRK-(DABCYL) | catalytic domain, 25°C, pH not specified in the publication | Homo sapiens | |
0.068 | - |
S80-glycosylated (EDANS)-ELAQAVRSSSRK-(DABCYL) | catalytic domain, 25°C, pH not specified in the publication | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P78536 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(EDANS)-ELAQAVRSSSRK-(DABCYL) + H2O | TNF-alpha derived substrate | Homo sapiens | ? | - |
? | |
(EDANS)-EPLAQAVRSSSK-(DABCYL) + H2O | TNF-alpha derived substrate | Homo sapiens | ? | - |
? | |
additional information | noncatalytic domains of ADAM17 do not directly bind to TNF-alpha derived substrates used but affect the binding nevertheless, most likely because of steric hindrance. Noncatalytic domains of ADAM17 affect the size/shape of the carbohydrate-binding pocket contained within the catalytic domain of ADAM17. Protease shows significantly decreased activity when the TNF-alpha-derived sequence is induced into alpha-helical conformation | Homo sapiens | ? | - |
? | |
S80-glycosylated (EDANS)-ELAQAVRSSSRK-(DABCYL) + H2O | TNF-alpha derived substrate | Homo sapiens | ? | - |
? | |
S80-glycosylated (EDANS)-EPLAQAVRSSSK-(DABCYL) + H2O | TNF-alpha derived substrate | Homo sapiens | ? | - |
? | |
TNF-alpha + H2O | - |
Homo sapiens | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
(EDANS)-ELAQAVRSSSRK-(DABCYL) | catalytic domain, 25°C, pH not specified in the publication | Homo sapiens | |
0.06 | - |
(EDANS)-EPLAQAVRSSSK-(DABCYL) | catalytic domain, presence of L-galactose, 25°C, pH not specified in the publication | Homo sapiens | |
0.07 | - |
(EDANS)-EPLAQAVRSSSK-(DABCYL) | catalytic domain, 25°C, pH not specified in the publication | Homo sapiens | |
0.08 | - |
S80-glycosylated (EDANS)-ELAQAVRSSSRK-(DABCYL) | catalytic domain, 25°C, pH not specified in the publication | Homo sapiens | |
0.08 | - |
S80-glycosylated (EDANS)-EPLAQAVRSSSK-(DABCYL) | extracellular domain, presence of L-galactose, 25°C, pH not specified in the publication | Homo sapiens | |
0.1 | - |
(EDANS)-EPLAQAVRSSSK-(DABCYL) | extracellular domain, presence of L-galactose, 25°C, pH not specified in the publication | Homo sapiens | |
0.14 | - |
(EDANS)-EPLAQAVRSSSK-(DABCYL) | extracellular domain, 25°C, pH not specified in the publication | Homo sapiens | |
0.2 | - |
(EDANS)-ELAQAVRSSSRK-(DABCYL) | extracellular domain, 25°C, pH not specified in the publication | Homo sapiens | |
0.22 | - |
S80-glycosylated (EDANS)-EPLAQAVRSSSK-(DABCYL) | catalytic domain, 25°C, pH not specified in the publication | Homo sapiens | |
0.25 | - |
S80-glycosylated (EDANS)-EPLAQAVRSSSK-(DABCYL) | extracellular domain, 25°C, pH not specified in the publication | Homo sapiens | |
0.36 | - |
S80-glycosylated (EDANS)-EPLAQAVRSSSK-(DABCYL) | catalytic domain, presence of L-galactose, 25°C, pH not specified in the publication | Homo sapiens | |
0.42 | - |
S80-glycosylated (EDANS)-ELAQAVRSSSRK-(DABCYL) | extracellular domain, 25°C, pH not specified in the publication | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
S80-glycosylated (EDANS)-ELAQAVRSSSRK-(DABCYL) | catalytic domain, 25°C, pH not specified in the publication | Homo sapiens | |
12 | - |
(EDANS)-EPLAQAVRSSSK-(DABCYL) | extracellular domain, 25°C, pH not specified in the publication | Homo sapiens | |
17 | - |
(EDANS)-EPLAQAVRSSSK-(DABCYL) | catalytic domain, 25°C, pH not specified in the publication | Homo sapiens | |
18 | - |
(EDANS)-EPLAQAVRSSSK-(DABCYL) | catalytic domain, presence of L-galactose, 25°C, pH not specified in the publication | Homo sapiens | |
19 | - |
(EDANS)-EPLAQAVRSSSK-(DABCYL) | extracellular domain, presence of L-galactose, 25°C, pH not specified in the publication | Homo sapiens | |
23 | - |
S80-glycosylated (EDANS)-EPLAQAVRSSSK-(DABCYL) | extracellular domain, presence of L-galactose, 25°C, pH not specified in the publication | Homo sapiens | |
32 | - |
S80-glycosylated (EDANS)-EPLAQAVRSSSK-(DABCYL) | catalytic domain, 25°C, pH not specified in the publication | Homo sapiens | |
36 | - |
S80-glycosylated (EDANS)-EPLAQAVRSSSK-(DABCYL) | catalytic domain, presence of L-galactose, 25°C, pH not specified in the publication | Homo sapiens | |
61 | - |
S80-glycosylated (EDANS)-ELAQAVRSSSRK-(DABCYL) | extracellular domain, 25°C, pH not specified in the publication | Homo sapiens | |
76 | - |
S80-glycosylated (EDANS)-EPLAQAVRSSSK-(DABCYL) | extracellular domain, 25°C, pH not specified in the publication | Homo sapiens |