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Literature summary for 3.4.24.86 extracted from

  • Stawikowska, R.; Cudic, M.; Giulianotti, M.; Houghten, R.; Fields, G.; Minond, D.
    Activity of ADAM17 (a disintegrin and metalloprotease 17) is regulated by its noncatalytic domains and secondary structure of its substrates (2013), J. Biol. Chem., 288, 22871-22879.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
S80-glycosylated (EDANS)-EPLAQAVRSSSK-(DABCYL) extracellular domain, 25°C, pH not specified in the publication Homo sapiens
0.0032
-
S80-glycosylated (EDANS)-EPLAQAVRSSSK-(DABCYL) extracellular domain, presence of L-galactose, 25°C, pH not specified in the publication Homo sapiens
0.0034
-
(EDANS)-EPLAQAVRSSSK-(DABCYL) catalytic domain, presence of L-galactose, 25°C, pH not specified in the publication Homo sapiens
0.004
-
(EDANS)-EPLAQAVRSSSK-(DABCYL) catalytic domain, 25°C, pH not specified in the publication Homo sapiens
0.004
-
(EDANS)-EPLAQAVRSSSK-(DABCYL) extracellular domain, presence of L-galactose, 25°C, pH not specified in the publication Homo sapiens
0.0069
-
S80-glycosylated (EDANS)-EPLAQAVRSSSK-(DABCYL) catalytic domain, 25°C, pH not specified in the publication Homo sapiens
0.0072
-
(EDANS)-ELAQAVRSSSRK-(DABCYL) extracellular domain, 25°C, pH not specified in the publication Homo sapiens
0.0076
-
S80-glycosylated (EDANS)-ELAQAVRSSSRK-(DABCYL) extracellular domain, 25°C, pH not specified in the publication Homo sapiens
0.01
-
S80-glycosylated (EDANS)-EPLAQAVRSSSK-(DABCYL) catalytic domain, presence of L-galactose, 25°C, pH not specified in the publication Homo sapiens
0.012
-
(EDANS)-EPLAQAVRSSSK-(DABCYL) extracellular domain, 25°C, pH not specified in the publication Homo sapiens
0.016
-
(EDANS)-ELAQAVRSSSRK-(DABCYL) catalytic domain, 25°C, pH not specified in the publication Homo sapiens
0.068
-
S80-glycosylated (EDANS)-ELAQAVRSSSRK-(DABCYL) catalytic domain, 25°C, pH not specified in the publication Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P78536
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(EDANS)-ELAQAVRSSSRK-(DABCYL) + H2O TNF-alpha derived substrate Homo sapiens ?
-
?
(EDANS)-EPLAQAVRSSSK-(DABCYL) + H2O TNF-alpha derived substrate Homo sapiens ?
-
?
additional information noncatalytic domains of ADAM17 do not directly bind to TNF-alpha derived substrates used but affect the binding nevertheless, most likely because of steric hindrance. Noncatalytic domains of ADAM17 affect the size/shape of the carbohydrate-binding pocket contained within the catalytic domain of ADAM17. Protease shows significantly decreased activity when the TNF-alpha-derived sequence is induced into alpha-helical conformation Homo sapiens ?
-
?
S80-glycosylated (EDANS)-ELAQAVRSSSRK-(DABCYL) + H2O TNF-alpha derived substrate Homo sapiens ?
-
?
S80-glycosylated (EDANS)-EPLAQAVRSSSK-(DABCYL) + H2O TNF-alpha derived substrate Homo sapiens ?
-
?
TNF-alpha + H2O
-
Homo sapiens ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.05
-
(EDANS)-ELAQAVRSSSRK-(DABCYL) catalytic domain, 25°C, pH not specified in the publication Homo sapiens
0.06
-
(EDANS)-EPLAQAVRSSSK-(DABCYL) catalytic domain, presence of L-galactose, 25°C, pH not specified in the publication Homo sapiens
0.07
-
(EDANS)-EPLAQAVRSSSK-(DABCYL) catalytic domain, 25°C, pH not specified in the publication Homo sapiens
0.08
-
S80-glycosylated (EDANS)-ELAQAVRSSSRK-(DABCYL) catalytic domain, 25°C, pH not specified in the publication Homo sapiens
0.08
-
S80-glycosylated (EDANS)-EPLAQAVRSSSK-(DABCYL) extracellular domain, presence of L-galactose, 25°C, pH not specified in the publication Homo sapiens
0.1
-
(EDANS)-EPLAQAVRSSSK-(DABCYL) extracellular domain, presence of L-galactose, 25°C, pH not specified in the publication Homo sapiens
0.14
-
(EDANS)-EPLAQAVRSSSK-(DABCYL) extracellular domain, 25°C, pH not specified in the publication Homo sapiens
0.2
-
(EDANS)-ELAQAVRSSSRK-(DABCYL) extracellular domain, 25°C, pH not specified in the publication Homo sapiens
0.22
-
S80-glycosylated (EDANS)-EPLAQAVRSSSK-(DABCYL) catalytic domain, 25°C, pH not specified in the publication Homo sapiens
0.25
-
S80-glycosylated (EDANS)-EPLAQAVRSSSK-(DABCYL) extracellular domain, 25°C, pH not specified in the publication Homo sapiens
0.36
-
S80-glycosylated (EDANS)-EPLAQAVRSSSK-(DABCYL) catalytic domain, presence of L-galactose, 25°C, pH not specified in the publication Homo sapiens
0.42
-
S80-glycosylated (EDANS)-ELAQAVRSSSRK-(DABCYL) extracellular domain, 25°C, pH not specified in the publication Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1
-
S80-glycosylated (EDANS)-ELAQAVRSSSRK-(DABCYL) catalytic domain, 25°C, pH not specified in the publication Homo sapiens
12
-
(EDANS)-EPLAQAVRSSSK-(DABCYL) extracellular domain, 25°C, pH not specified in the publication Homo sapiens
17
-
(EDANS)-EPLAQAVRSSSK-(DABCYL) catalytic domain, 25°C, pH not specified in the publication Homo sapiens
18
-
(EDANS)-EPLAQAVRSSSK-(DABCYL) catalytic domain, presence of L-galactose, 25°C, pH not specified in the publication Homo sapiens
19
-
(EDANS)-EPLAQAVRSSSK-(DABCYL) extracellular domain, presence of L-galactose, 25°C, pH not specified in the publication Homo sapiens
23
-
S80-glycosylated (EDANS)-EPLAQAVRSSSK-(DABCYL) extracellular domain, presence of L-galactose, 25°C, pH not specified in the publication Homo sapiens
32
-
S80-glycosylated (EDANS)-EPLAQAVRSSSK-(DABCYL) catalytic domain, 25°C, pH not specified in the publication Homo sapiens
36
-
S80-glycosylated (EDANS)-EPLAQAVRSSSK-(DABCYL) catalytic domain, presence of L-galactose, 25°C, pH not specified in the publication Homo sapiens
61
-
S80-glycosylated (EDANS)-ELAQAVRSSSRK-(DABCYL) extracellular domain, 25°C, pH not specified in the publication Homo sapiens
76
-
S80-glycosylated (EDANS)-EPLAQAVRSSSK-(DABCYL) extracellular domain, 25°C, pH not specified in the publication Homo sapiens