Literature summary for 3.4.24.85 extracted from

Schacherl, M.; Gompert, M.; Pardon, E.; Lamkemeyer, T.; Steyaert, J.; Baumann, U.
Crystallographic and biochemical characterization of the dimeric architecture of site-2 protease (2017), Biochim. Biophys. Acta Biomembr., 1859, 1859-1871 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli C43 (DE3) cells for full-length protein and BL21 (DE3) pLysS (DE3) for the CBS domain	Archaeoglobus fulgidus

Crystallization (Commentary)

Crystallization (Comment)	Organism
regulatory CBS domain pair of the enzyme in the apo and nucleotide-bound form, sitting drop vapor diffusion method, using 0.1 M Bis-Tris pH 6.5, 0.2 M (NH4)2SO4, 20% (w/v) PEG 3350	Archaeoglobus fulgidus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Archaeoglobus fulgidus	16020	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	contains zinc	Archaeoglobus fulgidus

Organism

Organism	UniProt	Comment	Textmining
Archaeoglobus fulgidus	O29915	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography, Strep-Tactin column chromatography, and Superdex S200 gel filtration	Archaeoglobus fulgidus

Subunits

Subunits	Comment	Organism
homodimer	x-ray crystallography	Archaeoglobus fulgidus

Synonyms

Synonyms	Comment	Organism
MEROPS:M50	-	Archaeoglobus fulgidus
S2P	-	Archaeoglobus fulgidus
site-2 protease	-	Archaeoglobus fulgidus

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Release 2026.1 (March 2026)