Literature summary for 3.4.24.85 extracted from

Zelenski, N.; Rawson, R.; Brown, M.; Goldstein, J.
Membrane topology of S2P, a protein required for intramembranous cleavage of sterol regulatory element-binding proteins (1999), J. Biol. Chem., 274, 21973-21980.

Search for more literature for 3.4.24.85

Application

Application	Comment	Organism
synthesis	in absence of cholesterol, SREBP double cleavage leads to activation of transcription of genes encoding multiple enzymes of the cholesterol biosynthetic pathway	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in M19 cell line	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
D467N	mutation of aspartate abolish activity suggesting that is the third residue that coordinates the zinc active site of S2P	Homo sapiens
N337Q	mutation at glycosylation site N337 does not abolish activity	Homo sapiens
N508Q	mutation does not abolish activity	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
SREBP NH2-522 fragment + H2O	Homo sapiens	-	NH2-484 fragment + 38 aa fragment	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	O43462	s2p cloned	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
HeLa cell	cDNA library	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
SREBP NH2-522 fragment + H2O	-	Homo sapiens	NH2-484 fragment + 38 aa fragment	-	?
SREBP NH2-522 fragment + H2O	cleavage at site-2 between 484-485 aa of SREBP cannot occur without prior cleavage at site-1 and is dependent on 478DRSR sequence	Homo sapiens	NH2-484 fragment + 38 aa fragment	-	?

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Release 2023.1 (January 2023)