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Literature summary for 3.4.24.83 extracted from
- Effects of metalloprotease anthrax lethal factor on its peptide-based inhibitor R9LF-1 (2015), Mol. Cell. Biochem., 406, 293-299 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 Star cells | Bacillus anthracis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| In2LF | i.e. Ac-Gly-Tyr-betaAla-(L-Arg)8-Val-Leu-Arg-CONHOH, competitive inhibitor | Bacillus anthracis |  |
| R9LF-1 | - |
Bacillus anthracis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| mitogen-activated protein kinase kinase + H2O | Bacillus anthracis | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus anthracis | P15917 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| His-Trap HP nickel affinity column chromatography | Bacillus anthracis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| In2LF + H2O | - |
Bacillus anthracis | In2LF fragments | - |
? | |
| mitogen-activated protein kinase kinase + H2O | - |
Bacillus anthracis | ? | - |
? | |
| R9LF-1 + H2O | - |
Bacillus anthracis | svR9LF-1 + NH2OH | - |
? | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.5 | the enzyme degrades R9LF-1 with maximum efficiency in the pH range of 7.0-8.5, which correlates well with the range of enzymatic activity with its native substrate | Bacillus anthracis |
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