Protein Variants | Comment | Organism |
---|---|---|
E269A | the mutant shows strongly increased cleavage ability for mitogen-activated protein kinase kinase-1 and reduced activity with mitogen-activated protein kinase kinase-6 compared to the wild type enzyme | Bacillus anthracis |
E539R | the mutant shows slightly increased cleavage ability for mitogen-activated protein kinase kinase-1 and reduced activity with mitogen-activated protein kinase kinase-6 compared to the wild type enzyme | Bacillus anthracis |
K75A | the mutant shows wild type activity | Bacillus anthracis |
L259A | the mutant cleaves MEK1 about 2fold less efficiently than the wild type enzyme | Bacillus anthracis |
L265A | the mutation does not significantly impact on MEK1 cleavage | Bacillus anthracis |
L431A | the mutant shows slightly increased cleavage ability for mitogen-activated protein kinase kinase-6 compared to the wild type enzyme | Bacillus anthracis |
M264A | the mutant cleaves MEK1 2.5fold less efficiently than the wild type enzyme | Bacillus anthracis |
R263A | the mutant shows reduced cleavage ability for mitogen-activated protein kinase kinase-1 compared to the wild type enzyme | Bacillus anthracis |
R491E | the mutant cleaves MEK1 about 2fold less efficiently than the wild type enzyme | Bacillus anthracis |
W271A | the mutation completely abolishes cleavage ability of mitogen-activated protein kinase kinase-6 but has no effect on the ability to cleave MEK1. The mutant blocks ERK phosphorylation and growth in a melanoma cell line, suggesting that it may provide a highly selective inhibitor of MEK1/2 for use as a cancer therapeutic | Bacillus anthracis |
Y268A | the mutant cleaves MEK1 6fold less efficiently than the wild type enzyme | Bacillus anthracis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
MEK-1 + H2O | Bacillus anthracis | - |
? | - |
? | |
MEK-2 + H2O | Bacillus anthracis | - |
? | - |
? | |
mitogen-activated protein kinase kinase-1 + H2O | Bacillus anthracis | - |
? | - |
? | |
mitogen-activated protein kinase kinase-3 + H2O | Bacillus anthracis | - |
? | - |
? | |
mitogen-activated protein kinase kinase-4 + H2O | Bacillus anthracis | - |
? | - |
? | |
mitogen-activated protein kinase kinase-6 + H2O | Bacillus anthracis | - |
? | - |
? | |
mitogen-activated protein kinase kinase-7 + H2O | Bacillus anthracis | - |
? | - |
? | |
NACHT leucine-rich repeat and pyrin domain-containing protein 1B + H2O | Bacillus anthracis | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus anthracis | P15917 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(7-methoxycoumarin-4-yl)acetyl-AKVYPYPME-(2,4-dinitrophenyldiaminopropionic acid) + H2O | - |
Bacillus anthracis | (7-methoxycoumarin-4-yl)acetyl-AKVYP + YPME-(2,4-dinitrophenyldiaminopropionic acid) | - |
? | |
MEK-1 + H2O | - |
Bacillus anthracis | ? | - |
? | |
MEK-2 + H2O | - |
Bacillus anthracis | ? | - |
? | |
mitogen-activated protein kinase kinase-1 + H2O | - |
Bacillus anthracis | ? | - |
? | |
mitogen-activated protein kinase kinase-3 + H2O | - |
Bacillus anthracis | ? | - |
? | |
mitogen-activated protein kinase kinase-4 + H2O | - |
Bacillus anthracis | ? | - |
? | |
mitogen-activated protein kinase kinase-6 + H2O | - |
Bacillus anthracis | ? | - |
? | |
mitogen-activated protein kinase kinase-7 + H2O | - |
Bacillus anthracis | ? | - |
? | |
NACHT leucine-rich repeat and pyrin domain-containing protein 1B + H2O | - |
Bacillus anthracis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 90000, SDS-PAGE | Bacillus anthracis |