Literature summary for 3.4.24.83 extracted from

Montpellier, L.; Siemann, S.
Effect of pH on the catalytic function and zinc content of native and immobilized anthrax lethal factor (2013), FEBS Lett., 587, 317-321.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Bacillus megaterium	Bacillus anthracis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	content is 1 mole per mole of protein. Zinc content is retained upon acidification up to a pH value of 5.5. At pH 5.3, release of about 40% of zinc, and below pH 4.5, sharp increase in the release of zinc	Bacillus anthracis

Organism

Organism	UniProt	Comment	Textmining
Bacillus anthracis	P15917	-	-

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.8	8	-	Bacillus anthracis

pH Range

pH Minimum	pH Maximum	Comment	Organism
4	-	inactive	Bacillus anthracis
5.8	-	progressive further loss of activity. Biphasic loss of activity upon acidification	Bacillus anthracis
6.7	-	about 60% of maximum activity. Biphasic loss of activity upon acidification	Bacillus anthracis

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
5	-	increase in protein aggreagation between pH 4 and pH 5	Bacillus anthracis

General Information

General Information	Comment	Organism
metabolism	about 30% demetallation is observed at pH values close to those found in late endosomes. A substantial proportion of lethal factor molecules may not be in their zinc-bound state prior to translocation	Bacillus anthracis

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Release 2023.1 (January 2023)