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Literature summary for 3.4.24.82 extracted from

  • Miwa, H.E.; Gerken, T.A.; Huynh, T.D.; Duesler, L.R.; Cotter, M.; Hering, T.M.
    Conserved sequence in the aggrecan interglobular domain modulates cleavage by ADAMTS-4 and ADAMTS-5 (2009), Biochim. Biophys. Acta, 1790, 161-172.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.82

Activating Compound

Activating Compound Comment Organism Structure
additional information substrate specificity of ADAMTS-4 against recombinant aggrecan, aggrecan mutants T370Q and T352V-T355V-T357V lead to a faster aggrecan cleavage Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information substrate specificity of ADAMTS-4 against recombinant aggrecan, aggrecan mutants V356A-V361A-E362D, V361Q-E362K, D360H-V361Q-E362K, S377Q lead to aggrecan cleavage inhibition Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Homo sapiens
-
 recombinant
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
aggrecan + H2O 2-8 microg, assay at pH 7.2, 37°C, reaction stopped by addition of 21 mM EDTA Homo sapiens ?
-
 ?
aggrecan + H2O non-conservative mutation at P2' position of the bovine aggrecan (R375L) does not result in an observable difference in ADAMTS-4 susceptibility. Mutations in recombinant bovine aggrecan at Ser377 to Thr or Asn do not result in any significant difference in ADAMTS-4 cleavage. The S377Q mutation results in a significant reduction of cleavage by ADAMTS-4. The S377Q mutant is susceptible to cleavage by the C-terminally truncated ADAMTS-4. The S377A mutant is cleaved similarly to wild-type aggrecan Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
ADAMTS-4
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Homo sapiens