Literature summary for 3.4.24.82 extracted from

Wayne, G.J.; Deng, S.J.; Amour, A.; Borman, S.; Matico, R.; Carter, H.L.; Murphy, G.
TIMP-3 inhibition of ADAMTS-4 (aggrecanase-1) is modulated by interactions between aggrecan and the C-terminal domain of ADAMTS-4 (2007), J. Biol. Chem., 282, 20991-20998.

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Cloned(Commentary)

Cloned (Comment)	Organism
the full-length mature form of ADAMTS-4 with a C-terminally His-tagged sequence is expressed in an Epstein-Barr virus nuclear antigen-expressing human embryonic kidney 293 cell line, two C-terminal domain deletion enzymes containing both catalytic and disintegrin domains and the catalytic domain alone are expressed in Drosophila S2 cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	the catalytic domain alone is enzymatically inactive	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
TIMP-3	-	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0203	-	Aggrecan	in 100 mM NaCl	Homo sapiens
0.0282	-	chondroitin 4-sulfate	in 100 mM NaCl	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography, Radial Q column chromatography, and heparin-Sepharose column chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
aggrecan + H2O	-	Homo sapiens	fragments of aggrecan	-	?
carboxyfluorescein-AELQGRPISIAK-N,N,N',N'-tetramethyl-6-carboxyrhodamine + H2O	-	Homo sapiens	carboxyfluorescein-AE + LQGRPISIAK-N,N,N',N'-tetramethyl-6-carboxyrhodamine	-	?
chondroitin 4-sulfate + H2O	-	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
a disintegrin and metalloproteinase with thrombospondin motifs-4	-	Homo sapiens
ADAMTS-4	-	Homo sapiens
aggrecanase 1	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.16	-	chondroitin 4-sulfate	in 100 mM NaCl	Homo sapiens
1.49	-	Aggrecan	in 100 mM NaCl	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0000079	-	TIMP-3	-	Homo sapiens

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Release 2023.1 (January 2023)