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Literature summary for 3.4.24.82 extracted from
- ADAMTS4 (aggrecanase-1) interaction with the C-terminal domain of fibronectin inhibits proteolysis of aggrecan (2004), J. Biol. Chem., 279, 32483-32491.
Application
| Application | Comment | Organism |
|---|---|---|
| degradation | degradation of cartilage proteoglycan (aggrecan) in osteoarthritis and rheumatoid arthritis | Bos taurus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Saccharomyces cerevisiae strain AH109 and in Escherichia coli strain DH5alpha | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| C-terminal 40-kDa fibronectin fragment | wild type enzyme IC50: 170 nM, complete inhibition at 750 nM | Bos taurus | |
| fibronectin | wild type enzyme IC50: 110 nM, complete inhibition at 500 nM | Bos taurus | |
| additional information | no inhibition of ADAMTS4 deletion mutants including DELTASp (DELTAArg693-Lys837, lacking the spacer domain) | Bos taurus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 73000 | - |
SDS-PAGE | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| SP-Sepharose fast flow column chromatography | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cartilage | - |
Bos taurus | - |
| chondrocyte | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| aggrecan + H2O | - |
Bos taurus | fragments of aggrecan | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ADAMTS4 | a disintegrin and metalloproteinase with thrombospondin motifs | Bos taurus |
| aggrecanase-1 | - |
Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Bos taurus |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00011 | - |
wild type enzyme IC50: 110 nM, complete inhibition at 500 nM | Bos taurus | fibronectin | |
| 0.00017 | - |
wild type enzyme IC50: 170 nM, complete inhibition at 750 nM | Bos taurus | C-terminal 40-kDa fibronectin fragment |
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Release 2023.1 (January 2023)
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