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Literature summary for 3.4.24.82 extracted from

  • Sugimoto, K.; Takahashi, M.; Yamamoto, Y.; Shimada, K.; Tanzawa, K.
    Identification of aggrecanase activity in medium of cartilage culture (1999), J. Biochem., 126, 449-455.
    View publication on PubMed
Search for more literature for 3.4.24.82

Inhibitors

Inhibitors Comment Organism Structure
actinonin 0.01 mg/ml, 61% inhibition Bos taurus
AG3340 0.00066 mM, 50% inhibition, hydroxamate-based matrix metalloproteinase inhibitor Bos taurus
BB94 0.000524 mM, 85% inhibition, hydroxamate-based matrix metalloproteinase inhibitor Bos taurus
chondroitin sulfate
-
 Bos taurus
CT-1746 0.000048 mM, 50% inhibition, hydroxamate-based matrix metalloproteinase inhibitor Bos taurus
EDTA 5 mM, complete inhibition Bos taurus
EGTA 5 mM, complete inhibition Bos taurus
glycosaminoglycan
-
 Bos taurus
heparin
-
 Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Ca2+-dependent metalloproteinase Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q Sepharose, Zn-chelating, ether Toyopearl, Mono S Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
articular cartilage
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 5300.0 mg/h/mg protein Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
aggrecan + H2O Glu373-Ala374 is the major cleavage site, aggrecanase also cleaves at Glu1971-Leu1972, which is located in the gap region in the chondroitin sulfate attachment region, aggrecanase does not cleave at the matrix metalloproteinase site Asn341-Phe342 Bos taurus fragments of aggrecan
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 ?