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Literature summary for 3.4.24.81 extracted from

  • Caescu, C.I.; Jeschke, G.R.; Turk, B.E.
    Active-site determinants of substrate recognition by the metalloproteinases TACE and ADAM10 (2009), Biochem. J., 424, 79-88.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
produced in insect cells by baculoviral expression as truncated constructs consisting of the signal sequence, pro- and catalytic domains fused to a C-terminal His6 (hexahistidine) tag (residues 1–460 of mouse ADAM10). Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information peptide libraries are used to define the cleavage site selectivity of TACE (EC 3.4.24.86) and ADAM10. The two proteases have distinct primary sequence requirements at multiple positions surrounding the cleavage site in their substrates, which allows to generate peptide substrates that are highly specific for each of these proteases. The major difference between the two protease specificities maps to the P1' position (immediately downstream of the cleavage site) of the substrate. At this position, TACE is selective for smaller aliphatic residues, whereas ADAM10 can accommodate aromatic amino acids. Using mutagenesis three residues in the S1' pockets of these enzymes are identified that dramatically influence specificity for both peptide and protein substrates Mus musculus ?
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Synonyms

Synonyms Comment Organism
ADAM10
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Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
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assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
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assay at Mus musculus

General Information

General Information Comment Organism
physiological function ADAM10 is a primary enzymes responsible for catalysing release of membrane-anchored proteins from the cell surface in metazoan organisms Mus musculus