Cloned (Comment) | Organism |
---|---|
a C-terminal HA-tagged fusion protein is transfected in human ovarian cancer SKOV3 cells and human embryonic kidney HEK293 cells | Homo sapiens |
a C-terminal HA-tagged fusion protein is transfected in human ovarian cancer SKOV3 cells and human embryonic kidney HEK293 cells | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
S269A | mutant is detected at lower molecular masses than the wild-type form, which indicates that the N-glycosylation site is occupied | Bos taurus |
S441A | mutant is detected at lower molecular masses than the wild-type form, which indicates that the N-glycosylation site is occupied, mutant shows increased ADAM10 susceptibility to proteolysis | Bos taurus |
T280A | mutant is detected at lower molecular masses than the wild-type form, which indicates that the N-glycosylation site is occupied, T280A is found to accumulate in the endoplasmic reticulum as the non-processed precursor of the enzyme. Mutant exhibits only residual levels of metalloprotease activity | Bos taurus |
T553A | mutant is detected at lower molecular masses than the wild-type form, which indicates that the N-glycosylation site is occupied | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endosome | - |
Bos taurus | 5768 | - |
perinuclear space | predominant localization of the protein at the perinuclear region and at the cell surface where it appears as punctuated dots evenly distributed at the plasma membrane | Homo sapiens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
Western blot, non-reducing conditions | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | contained high-mannose as well as complex-type N-glycans | Homo sapiens |
glycoprotein | contains high-mannose as well as complex-type N-glycans. Glycosylation sites: S269, T280, S441, T553 | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
SKOV-3 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Abz-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Arg-Dap(dnp)-NH2 + H2O | - |
Homo sapiens | ? | - |
? | |
Abz-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Arg-Dap(dnp)-NH2 + H2O | - |
Bos taurus | ? | - |
? | |
L1 cell adhesion molecule + H2O | - |
Bos taurus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADAM10 | - |
Homo sapiens |
ADAM10 | - |
Bos taurus |