Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.80 extracted from

  • Butler, G.S.; Connor, A.R.; Sounni, N. E.; Eckhard, U.; Morrison, C.J.; Noel, A.; Overall, C.M.
    Degradomic and yeast 2-hybrid inactive catalytic domain substrate trapping identifies new membrane-type 1 matrix metalloproteinase (MMP14) substrates CCN3 (Nov) and CCN5 (WISP2) (2017), Matrix Biol., 59, 23-38 .
    View publication on PubMed
Search for more literature for 3.4.24.80

Cloned(Commentary)

Cloned (Comment) Organism
gene MMP14, recombinant coexpression of MMP14 with CCN5 in MCF-7 and CHO-K1 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
E240A inactive MMP14 mutant Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
 Homo sapiens 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ a zinc-dependent endopeptidase Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CCN3 + H2O Homo sapiens
-
 ?
-
 ?
CCN5 + H2O Homo sapiens UniProt ID O76076, CCN5 lacks the CTCK domain ?
-
 ?
additional information Homo sapiens interaction analysis of MMP14 with proteins in HeLa cells identified by inactive catalytic domain capture yeast 2-hybrid analysis, overview. Detection of CCN5 as a MMP14 substrate by yeast 2-hybrid screening ?
-
 ?
proMMP-2 + H2O Homo sapiens
-
 MMP-2 + MMP-2 pro-peptide
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P50281
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CCN3 + H2O
-
 Homo sapiens ?
-
 ?
CCN3 + H2O
-
 Homo sapiens CPPQCPGR + DGQIGCVPR + KVEVPGECCEK + KPVMVIGTCTCHTNCPK + ?
-
 ?
CCN5 + H2O
-
 Homo sapiens ?
-
 ?
CCN5 + H2O UniProt ID O76076, CCN5 lacks the CTCK domain Homo sapiens ?
-
 ?
additional information interaction analysis of MMP14 with proteins in HeLa cells identified by inactive catalytic domain capture yeast 2-hybrid analysis, overview. Detection of CCN5 as a MMP14 substrate by yeast 2-hybrid screening Homo sapiens ?
-
 ?
additional information CCN = cysteine rich protein 61/connective tissue growth factor/nephroblastoma overexpressed Homo sapiens ?
-
 ?
proMMP-2 + H2O
-
 Homo sapiens MMP-2 + MMP-2 pro-peptide
-
 ?

Synonyms

Synonyms Comment Organism
membrane-type 1 matrix metalloproteinase
-
 Homo sapiens
MMP14
-
 Homo sapiens
MT1-MMP
-
 Homo sapiens

Expression

Organism Comment Expression
Homo sapiens MCF-7 cells are transfected with human CCN5 using a polyclonal anti-VTSP antibody detected only full length CCN5. CCN5 cleavage occurs upon coexpression of MMP14 with CCN5 in the MCF-7 cells resulting in the loss of the VTSP epitope in the medium. Cleavage of full-length CCN5 in the CHO-K1-conditioned medium upon addition of recombinant MMP14 or MMP2 additional information

General Information

General Information Comment Organism
metabolism CCN5 (WISP2) is processed in the variable region by MMP14 and MMP2, as well as by MMP1, 3, 7, 8, 9 and 15. CCN5 cleavage by proangiogenic MMPs results in removal of an angiogenic brake held by CCN5. CCN3 is processed most efficiently by MMP14, 2 and 1 from about 30 kDa to fragments of 20-25 kDa, and less efficiently by MMP8 and 9 Homo sapiens
additional information the catalytic domain comprises residues Tyr112-Gly284 Homo sapiens
physiological function MMP14 is a regulator of angiogenesis, it cleaves CCN5 and abrogates the angiostatic activity. CCN5 is an angiogenesis inhibitor that is inactivated by MMP14 cleavage. Proteolytic processing of CCN3 by MMP14 Homo sapiens