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Literature summary for 3.4.24.80 extracted from

  • Ogata, H.; Decaneto, E.; Grossman, M.; Havenith, M.; Sagi, I.; Lubitz, W.; Knipp, M.
    Crystallization and preliminary X-ray crystallographic analysis of the catalytic domain of membrane type 1 matrix metalloproteinase (2014), Acta Crystallogr. Sect. F, 70, 232-235.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.80

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.1 M sodium malonate pH 4.0, 20% (w/v) PEG 3350, or 4% (v/v) tacsimate pH 6.0, 12% (w/v) PEG 3350, or 0.2 M ammonium nitrate, 20% (w/v) PEG 3350 pH 6.2 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ dependent on Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
 x * 21000, catalytic domain, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P50281
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
dialysis against a 10fold volume of 50 mM Tris-HCl pH 8.5, 100 mM NaCl, 10 mM CaCl2, 10 mM zinc acetate, and 2 M urea Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(7-methoxycoumarin-4-yl)-acetyl-L-Lys-Pro-Leu-Gly-Leu-Lys(N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl)-Ala-Arg-NH2 + H2O
-
 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 21000, catalytic domain, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
membrane type 1 matrix metalloproteinase
-
 Homo sapiens
MT1-MMP
-
 Homo sapiens