Application | Comment | Organism |
---|---|---|
medicine | MT1-MMP initiates substrate degradation and enhances cell migration, hemopexin-like domain and a nonenzymatic component of the catalytic domain of MT1-MMP are essential for MT1-MMP-mediated cell migration, Rac1 participates in the MT1-MMP signal transduction pathway | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
cloned into pSG5 vector, expression in COS-1 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E240A | expressed on transfected cell surface but fails to activate pMMP-2 | Homo sapiens |
K44A | dominant negative dynamin mutation controlled by a separate cytomegalovirus promoter MT1/K44A, leading to increased substrate digestion that is contributed by enhanced cell migration resulting from the accumulation of MT1-MMP ant the plasma membrane | Homo sapiens |
additional information | mutant Sol.MT, fails to activate pMMP-2, mutant MTdeltaC, pMMP-2 activation as compared with wild type, neither cell type-dependent nor extracellular matrix component-dependent | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | cells cotransfected with TIMP-1 cDNA along with MT1-MMP and pMMP-2 cDNAs result in partial inhibition of substrate degradation, down to the basal digestion level resulting from MT1-MMP alone, wortmannin and PD98059 do not interfer with MT1-MMP-induced cell migration, CDC42 (N17) and RhoA(N19) have no effect on MT1-MMP-dependent cell migration | Homo sapiens | |
Rac1(N17Rac) | coexpression wit MT1-MMP cDNAs leads to complete inhibition of migration | Homo sapiens | |
TIMP-2 | totally abolishes substrate degradation | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | short cytoplasmic tail | Homo sapiens | 5737 | - |
perinuclear space | highest amounts | Homo sapiens | - |
- |
plasma membrane | plasma membrane anchor | Homo sapiens | 5886 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
MT1-GFP fusion protein, Western blot analysis | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
breast cancer cell line | - |
Homo sapiens | - |
LNCaP cell | - |
Homo sapiens | - |
MDA-MB-231 cell | - |
Homo sapiens | - |
prostate cancer cell line | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fibrin + H2O | - |
Homo sapiens | ? | - |
? | |
Fibrinogen + H2O | - |
Homo sapiens | ? | - |
? | |
Fibronectin + H2O | cell surface active pMMP-2 cDNAs bound to MT1-MMP enhances substrate digestion, cytoplasmic tail of MT1-MMP not required for digestion | Homo sapiens | ? | - |
? | |
Gelatin + H2O | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
membrane type 1 matrix metalloproteinase | - |
Homo sapiens |
MT1-MMP | - |
Homo sapiens |