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Literature summary for 3.4.24.80 extracted from

  • Rozanov, D.V.; Golubkov, V.S.; Strongin, A.Y.
    Membrane type-1 matrix metalloproteinase (MT1-MMP) protects malignant cells from tumoricidal activity of re-engineered anthrax lethal toxin (2005), Int. J. Biochem. Cell Biol., 37, 142-154.
    View publication on PubMed
Search for more literature for 3.4.24.80

Activating Compound

Activating Compound Comment Organism Structure
furin involved in the activation of MT1-MMP Homo sapiens

Application

Application Comment Organism
medicine MT1-MMP proteolysis of protective antigen markers makes the MT1-MMP-expressing aggressive invasive cells resistant to the cytotoxic effect of bipartite PA/FP59 toxin, synthetic inhibitors of MMPs are likely to increase the therapeutic anti-cancer effect of anthrax toxin, unique role of furin in the activation of protective antigen markers Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
catalytic domain of MT1-MMP expressed in Escherichia coli, MT1-MMP cDNAs cloned into vector pcDNA3-zeo Homo sapiens

Protein Variants

Protein Variants Comment Organism
E240A catalytically inert Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
AB815 against hinge region of MT1-MMP Homo sapiens
decanoyl-Arg-Val-Lys-Arg-chloromethylketone furin inhibitor repressing activation of MMP-2 Homo sapiens
GM6001 fully blocks proteolysis of PA83 by MT1-MMP, inhibits MT1-MMP-dependent activation of proMMP-2 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 Western blot analysis, stable, catalytically inactive ectodomain form Homo sapiens
42000
-
 Western blot analysis, stable, catalytically inactive ectodomain form Homo sapiens
60000
-
 Western blot analysis Homo sapiens
63000
-
 Western blot analysis, proenzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purified from exclusion bodies Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HT-1080 cell stably transfected with wild type MT1-MMP Homo sapiens
-
LoVo cell furin-negative cells stably expressing wild type MT1-MMP and reconstituted furin-positive cells Homo sapiens
-
additional information mock-transfected control cells Homo sapiens
-
U-251 cell stably expresses wild type MT1-MMP Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
PA83 efficiently cleaved by MT1-MMP at the substrate-enzyme ratio as low as 1: 50 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
membrane type-1 matrix metalloproteinase
-
 Homo sapiens
MT1-MMP
-
 Homo sapiens