Cloned (Comment) | Organism |
---|---|
18 kDa inactive fragment ranging from Tyr 112 to Ala 255, 21 kDa fragment ranging from Tyr 122 to Gly 284 and truncated form lacking the cytosolic domain | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E240A | catalytically inactive | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
marimastat | inhibits production of 18 kDa fragment during autocatalytic processing | Homo sapiens | |
TIMP-2 | inhibits production of 18 kDa fragment during autocatalytic processing | Homo sapiens | |
TIMP-4 | inhibits production of 18 kDa fragment during autocatalytic processing | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
MOCAcPLGLA2pr-dinitrophenol-A-RNH2 | pH 7.5, Tyr112-Gly284 fragment | Homo sapiens | |
0.01 | - |
MOCAcPLGLA2pr-dinitrophenol-A-RNH2 | pH 7.5, Tyr112-Gly288 fragment | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
570000 | - |
active form, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P50281 | - |
- |
Purification (Comment) | Organism |
---|---|
18 kDa and 21 kDa fragment, homogeneity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
MOCAcPLGLA2pr-dinitrophenol-A-RNH2 + H2O | fluorogenic substrate | Homo sapiens | ? | - |
? | |
progelatinase A + H2O | 21 kDa fragment | Homo sapiens | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
MOCAcPLGLA2pr-dinitrophenol-A-RNH2 | pH 7.5, Tyr112-Gly288 fragment | Homo sapiens | |
0.055 | - |
MOCAcPLGLA2pr-dinitrophenol-A-RNH2 | pH 7.5, Tyr112-Gly284 fragment | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000005 | - |
TIMP-2 | Tyr112-Gly288 fragment | Homo sapiens | |
0.00000006 | - |
TIMP-2 | Tyr112-Gly288 fragment | Homo sapiens |