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Literature summary for 3.4.24.79 extracted from
- Definition, expression, and characterization of a protein domain in the N-terminus of pregnancy-associated plasma protein-A distantly related to the family of laminin G-like modules (2006), Protein Expr. Purif., 48, 261-273.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the His6-tagged PAPP-A laminin G domain in Escherichia coli, expression of wild-type andtruncation mutant enzymes, comparising residues 1-310, in HEK293T cells | Homo sapiens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 400000 | - |
- |
Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| IGF-binding protein-4 + H2O | Homo sapiens | i.e. IGFBP-4 | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged PAPP-A laminin G domain from Escherichia coli by nickel affinity chromatography | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| IGF-binding protein-4 + H2O | i.e. IGFBP-4 | Homo sapiens | ? | - |
? | |
| IGF-binding protein-5 + H2O | i.e. IGFBP-5 | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | homodimer, a laminin G-like module is contained within the N-terminal region, secondary structure prediction and sequence alignment, overview | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| IGFBP proteinase | - |
Homo sapiens |
| insulin-like growth factor-binding protein proteinase | - |
Homo sapiens |
| More | PAPP-A and PAPP-A2 are unique members in the group of laminin G proteins, the pappalysins represent examples where LG modules are associated with proteinases | Homo sapiens |
| PAPP-A | - |
Homo sapiens |
| pregnancy-associated plasma protein-A | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
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Release 2023.1 (January 2023)
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