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Literature summary for 3.4.24.77 extracted from

  • Vitale, L.; Vukelic, B.; Krizaj, I.
    Extracellular metalloendopeptidase of Streptomyces rimosus (2006), Arch. Microbiol., 185, 183-191.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 1 mM, complete inhibition Streptomyces rimosus
EDTA 1 mM, complete inhibition Streptomyces rimosus
additional information no inhibitory effect of phenyl-methyl-sulfonyl-fluoride and p-chlormercuribenzoate Streptomyces rimosus
N-bromosuccinimide 1 mM, 97% inhibition Streptomyces rimosus
thiorphan 0.001 mM and 0.0001 mM, 35% and 47% inhibition, respectively Streptomyces rimosus
Zn2+ 0.01 mM Streptomyces rimosus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 0.1-1 mM, up to 3 old increase of activity Streptomyces rimosus
Co2+ 0.1-1 mM, up to 3fold increase of activity Streptomyces rimosus
Mn2+ 0.1-1 mM, up to 3fold increase of activity Streptomyces rimosus
Zn2+ 0.001 mM Streptomyces rimosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15000
-
SDS-PAGE Streptomyces rimosus
16000
-
SDS-PAGE Streptomyces rimosus

Organism

Organism UniProt Comment Textmining
Streptomyces rimosus
-
-
-
Streptomyces rimosus K-OK-02
-
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration and ion exchange chromatography Streptomyces rimosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
no degradation of Bz-L-Arg-2-naphthylamide, Bz-L-Tyr-2-naphthylamide, fyrylacryloyl-Gly-Leu-NH2, Z-Gly-Gly-Leu-p-nitroanilide Streptomyces rimosus
4.6
-
Suc-Ala-Ala-Phe-p-nitroanilide as substrate Streptomyces rimosus
5.3
-
bovine serum albumin as substrate Streptomyces rimosus
5.7
-
Gly-Gly-Gly-Phe-2-naphthylamide as substrate Streptomyces rimosus
6.4
-
beta-lactoglobulin as substrate Streptomyces rimosus
30.4
-
casein as substrate Streptomyces rimosus
33.3
-
gelatin as substrate Streptomyces rimosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Arg-Gly-Phe-Phe + H2O
-
Streptomyces rimosus Arg-Gly + Phe-Phe
-
?
Arg-Gly-Pro-Phe-Pro-Ile + H2O
-
Streptomyces rimosus Arg-Gly-Pro + Phe-Pro-Ile
-
?
Arg-Pro-Pro-Gly-Phe-Ser-Pro + H2O
-
Streptomyces rimosus Arg-Pro-Pro-Gly + Phe-Ser-Pro
-
?
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O
-
Streptomyces rimosus Arg-Pro-Pro-Gly-Phe-Ser-Pro + Phe-Arg
-
?
Azocoll + H2O
-
Streptomyces rimosus ?
-
?
Azocoll + H2O
-
Streptomyces rimosus K-OK-02 ?
-
?
Bovine serum albumin + H2O
-
Streptomyces rimosus ?
-
?
Bovine serum albumin + H2O
-
Streptomyces rimosus K-OK-02 ?
-
?
casein + H2O
-
Streptomyces rimosus ?
-
?
casein + H2O
-
Streptomyces rimosus K-OK-02 ?
-
?
furylacryloyl-Met-Leu-Phe-Lys + H2O
-
Streptomyces rimosus furylacryloyl-Met-Leu + Phe-Lys
-
?
Gelatin + H2O
-
Streptomyces rimosus ?
-
?
Glucagon + H2O
-
Streptomyces rimosus ?
-
?
Glucagon + H2O
-
Streptomyces rimosus K-OK-02 ?
-
?
Gly-Gly-Phe-Leu + H2O
-
Streptomyces rimosus Gly-Gly + Phe-Leu
-
?
Insulin B-chain + H2O
-
Streptomyces rimosus ?
-
?
Insulin B-chain + H2O
-
Streptomyces rimosus K-OK-02 ?
-
?
Leu-Trp-Met-Arg-Phe + H2O
-
Streptomyces rimosus Leu-Trp + Met-Arg-Phe
-
?
Leu-Trp-Met-Arg-Phe-Ala + H2O
-
Streptomyces rimosus Leu-Trp + Met-Arg + Phe-Ala
-
?
Met-Arg-Phe-Ala + H2O
-
Streptomyces rimosus Met-Arg + Phe-Ala
-
?
Met-Gly-Trp-Met-Asp-Phe-NH2 + H2O
-
Streptomyces rimosus Met-Gly-Trp-Met-Asp + Phe-NH2
-
?
Phe-Phe-Phe-Phe + H2O
-
Streptomyces rimosus Phe-Phe + Phe-Phe
-
?
Tyr-Gly-Gly-Phe-Leu + H2O
-
Streptomyces rimosus Tyr-Gly-Gly + Phe-Leu
-
?
Tyr-Gly-Gly-Phe-Met + H2O
-
Streptomyces rimosus Tyr-Gly-Gly + Phe-Met
-
?
Tyr-Pro-Phe-Pro + H2O
-
Streptomyces rimosus Tyr-Pro + Phe-Pro
-
?

Synonyms

Synonyms Comment Organism
extracellular metalloendopeptidase
-
Streptomyces rimosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Streptomyces rimosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
during 30 min of various temperatures the enzyme is stable until 30°C, but progressively lost its activity at higher temperature Streptomyces rimosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Streptomyces rimosus

pH Range

pH Minimum pH Maximum Comment Organism
7.4 7.9
-
Streptomyces rimosus

pI Value

Organism Comment pI Value Maximum pI Value
Streptomyces rimosus
-
-
9.5