Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 1 mM, complete inhibition | Streptomyces rimosus | |
EDTA | 1 mM, complete inhibition | Streptomyces rimosus | |
additional information | no inhibitory effect of phenyl-methyl-sulfonyl-fluoride and p-chlormercuribenzoate | Streptomyces rimosus | |
N-bromosuccinimide | 1 mM, 97% inhibition | Streptomyces rimosus | |
thiorphan | 0.001 mM and 0.0001 mM, 35% and 47% inhibition, respectively | Streptomyces rimosus | |
Zn2+ | 0.01 mM | Streptomyces rimosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 0.1-1 mM, up to 3 old increase of activity | Streptomyces rimosus | |
Co2+ | 0.1-1 mM, up to 3fold increase of activity | Streptomyces rimosus | |
Mn2+ | 0.1-1 mM, up to 3fold increase of activity | Streptomyces rimosus | |
Zn2+ | 0.001 mM | Streptomyces rimosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15000 | - |
SDS-PAGE | Streptomyces rimosus |
16000 | - |
SDS-PAGE | Streptomyces rimosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces rimosus | - |
- |
- |
Streptomyces rimosus K-OK-02 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
gel filtration and ion exchange chromatography | Streptomyces rimosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
no degradation of Bz-L-Arg-2-naphthylamide, Bz-L-Tyr-2-naphthylamide, fyrylacryloyl-Gly-Leu-NH2, Z-Gly-Gly-Leu-p-nitroanilide | Streptomyces rimosus |
4.6 | - |
Suc-Ala-Ala-Phe-p-nitroanilide as substrate | Streptomyces rimosus |
5.3 | - |
bovine serum albumin as substrate | Streptomyces rimosus |
5.7 | - |
Gly-Gly-Gly-Phe-2-naphthylamide as substrate | Streptomyces rimosus |
6.4 | - |
beta-lactoglobulin as substrate | Streptomyces rimosus |
30.4 | - |
casein as substrate | Streptomyces rimosus |
33.3 | - |
gelatin as substrate | Streptomyces rimosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Arg-Gly-Phe-Phe + H2O | - |
Streptomyces rimosus | Arg-Gly + Phe-Phe | - |
? | |
Arg-Gly-Pro-Phe-Pro-Ile + H2O | - |
Streptomyces rimosus | Arg-Gly-Pro + Phe-Pro-Ile | - |
? | |
Arg-Pro-Pro-Gly-Phe-Ser-Pro + H2O | - |
Streptomyces rimosus | Arg-Pro-Pro-Gly + Phe-Ser-Pro | - |
? | |
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O | - |
Streptomyces rimosus | Arg-Pro-Pro-Gly-Phe-Ser-Pro + Phe-Arg | - |
? | |
Azocoll + H2O | - |
Streptomyces rimosus | ? | - |
? | |
Azocoll + H2O | - |
Streptomyces rimosus K-OK-02 | ? | - |
? | |
Bovine serum albumin + H2O | - |
Streptomyces rimosus | ? | - |
? | |
Bovine serum albumin + H2O | - |
Streptomyces rimosus K-OK-02 | ? | - |
? | |
casein + H2O | - |
Streptomyces rimosus | ? | - |
? | |
casein + H2O | - |
Streptomyces rimosus K-OK-02 | ? | - |
? | |
furylacryloyl-Met-Leu-Phe-Lys + H2O | - |
Streptomyces rimosus | furylacryloyl-Met-Leu + Phe-Lys | - |
? | |
Gelatin + H2O | - |
Streptomyces rimosus | ? | - |
? | |
Glucagon + H2O | - |
Streptomyces rimosus | ? | - |
? | |
Glucagon + H2O | - |
Streptomyces rimosus K-OK-02 | ? | - |
? | |
Gly-Gly-Phe-Leu + H2O | - |
Streptomyces rimosus | Gly-Gly + Phe-Leu | - |
? | |
Insulin B-chain + H2O | - |
Streptomyces rimosus | ? | - |
? | |
Insulin B-chain + H2O | - |
Streptomyces rimosus K-OK-02 | ? | - |
? | |
Leu-Trp-Met-Arg-Phe + H2O | - |
Streptomyces rimosus | Leu-Trp + Met-Arg-Phe | - |
? | |
Leu-Trp-Met-Arg-Phe-Ala + H2O | - |
Streptomyces rimosus | Leu-Trp + Met-Arg + Phe-Ala | - |
? | |
Met-Arg-Phe-Ala + H2O | - |
Streptomyces rimosus | Met-Arg + Phe-Ala | - |
? | |
Met-Gly-Trp-Met-Asp-Phe-NH2 + H2O | - |
Streptomyces rimosus | Met-Gly-Trp-Met-Asp + Phe-NH2 | - |
? | |
Phe-Phe-Phe-Phe + H2O | - |
Streptomyces rimosus | Phe-Phe + Phe-Phe | - |
? | |
Tyr-Gly-Gly-Phe-Leu + H2O | - |
Streptomyces rimosus | Tyr-Gly-Gly + Phe-Leu | - |
? | |
Tyr-Gly-Gly-Phe-Met + H2O | - |
Streptomyces rimosus | Tyr-Gly-Gly + Phe-Met | - |
? | |
Tyr-Pro-Phe-Pro + H2O | - |
Streptomyces rimosus | Tyr-Pro + Phe-Pro | - |
? |
Synonyms | Comment | Organism |
---|---|---|
extracellular metalloendopeptidase | - |
Streptomyces rimosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Streptomyces rimosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
during 30 min of various temperatures the enzyme is stable until 30°C, but progressively lost its activity at higher temperature | Streptomyces rimosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Streptomyces rimosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.4 | 7.9 | - |
Streptomyces rimosus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Streptomyces rimosus | - |
- |
9.5 |