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Literature summary for 3.4.24.75 extracted from

  • Mitkowski, P.; Jagielska, E.; Nowak, E.; Bujnicki, J.M.; Stefaniak, F.; Niedzialek, D.; Bochtler, M.; Sabala, I.
    Structural bases of peptidoglycan recognition by lysostaphin SH3b domain (2019), Sci. Rep., 9, 5965 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Staphylococcus simulans

Crystallization (Commentary)

Crystallization (Comment) Organism
lysostaphin SH3b domain in complex with a pentaglycine peptide, hanging drop vapor diffusion method Staphylococcus simulans

Protein Variants

Protein Variants Comment Organism
N405A the mutation does not affect lytic activity of the mature enzyme Staphylococcus simulans
N405A/Y472A the mutations do not affect lytic activity of the mature enzyme Staphylococcus simulans
Y472A the mutation does not affect lytic activity of the mature enzyme Staphylococcus simulans

Organism

Organism UniProt Comment Textmining
Staphylococcus simulans P10547
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap column chromatography and Superdex 75gel filtration Staphylococcus simulans

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Staphylococcus aureus mutant strain femAB cell wall + H2O
-
Staphylococcus simulans ?
-
?
Staphylococcus aureus strain TF5311 cell wall + H2O
-
Staphylococcus simulans ?
-
?

Synonyms

Synonyms Comment Organism
SH3b cell wall binding domain Staphylococcus simulans