Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.75 extracted from

  • Duman, Z.E.; Uenlue, A.; Cakar, M.M.; Uenal, H.; Binay, B.
    Enhanced production of recombinant Staphylococcus simulans lysostaphin using medium engineering (2019), Prep. Biochem. Biotechnol., 49, 521-528 .
    View publication on PubMed
Search for more literature for 3.4.24.75

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Top10 cells Staphylococcus simulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Staphylococcus simulans the enzyme hydrolyzes the glycylglycine bonds of polyglycine interpeptide bridges on the cell walls of Staphylococcus aureus, Staphylococcus epidermidis and Staphylococcus carnosus ?
-
 ?
additional information Staphylococcus simulans NRRLB-2628 the enzyme hydrolyzes the glycylglycine bonds of polyglycine interpeptide bridges on the cell walls of Staphylococcus aureus, Staphylococcus epidermidis and Staphylococcus carnosus ?
-
 ?
Staphylococcus aureus ATCC 29213 cells + H2O Staphylococcus simulans
-
 ?
-
 ?
Staphylococcus aureus ATCC 29213 cells + H2O Staphylococcus simulans NRRLB-2628
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Staphylococcus simulans P10547 biovar staphylolyticus
-
Staphylococcus simulans NRRLB-2628 P10547 biovar staphylolyticus
-

Purification (Commentary)

Purification (Comment) Organism
TAGZyme purification system Staphylococcus simulans

Storage Stability

Storage Stability Organism
-80°C, recombinant enzyme in 100 mM Tris-HCl pH 7.4, 30 and 40 days, the enzyme loses approximately 25% and 40% of its activity, respectively Staphylococcus simulans
4°C and 25°C, recombinant enzyme in 100 mM Tris-HCl pH 7.4, 15 days, the enzyme loses approximately half of its activity Staphylococcus simulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme hydrolyzes the glycylglycine bonds of polyglycine interpeptide bridges on the cell walls of Staphylococcus aureus, Staphylococcus epidermidis and Staphylococcus carnosus Staphylococcus simulans ?
-
 ?
additional information the enzyme hydrolyzes the glycylglycine bonds of polyglycine interpeptide bridges on the cell walls of Staphylococcus aureus, Staphylococcus epidermidis and Staphylococcus carnosus Staphylococcus simulans NRRLB-2628 ?
-
 ?
Staphylococcus aureus ATCC 29213 cells + H2O
-
 Staphylococcus simulans ?
-
 ?
Staphylococcus aureus ATCC 29213 cells + H2O
-
 Staphylococcus simulans NRRLB-2628 ?
-
 ?