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Literature summary for 3.4.24.75 extracted from
- High level activity of recombinant lysostaphin after computer simulation and additive-based refolding (2019), Int. J. Pept. Res. Ther., 25, 1241-1249 .
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 (DE3) pLysS cells | Staphylococcus simulans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus simulans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Staphylococcus simulans |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| a buffer containing proline 0.15 M and glucose 0.2 M causes the best enzyme refolding | Staphylococcus simulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methicillin-resistant Staphylococcus aureus cells + H2O | - |
Staphylococcus simulans | ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 27000, recombiannt enzyme, SDS-PAGE | Staphylococcus simulans |
| ? | x * 42000, pET32a-tagged enzyme, SDS-PAGE | Staphylococcus simulans |
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Release 2023.1 (January 2023)
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