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Literature summary for 3.4.24.75 extracted from

  • Wu, X.; Kwon, S.J.; Kim, D.; Zha, J.; Mora-Pale, M.; Dordick, J.S.
    Unprotonated short-chain alkylamines inhibit staphylolytic activity of lysostaphin in a wall teichoic acid-dependent manner (2018), Appl. Environ. Microbiol., 84, e00693-18 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Star (DE3) cells Staphylococcus simulans

Inhibitors

Inhibitors Comment Organism Structure
diethanolamine
-
 Staphylococcus simulans
ethanolamine inhibits enzyme binding to the cell surface, leading to low enzyme activity, despite retention of its secondary structure. At pH 10.5 (but not at pH 7.5), methanolamine strongly deactivates the enzyme in a concentration-dependent manner Staphylococcus simulans
ethylenediamine
-
 Staphylococcus simulans
triethanolamine
-
 Staphylococcus simulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Staphylococcus aureus cell wall + H2O Staphylococcus simulans Staphylococcus aureus ATCC 6538 or ATCC 33807 cells ?
-
 ?

Organism

Organism UniProt Comment Textmining
Staphylococcus simulans
-
 biovar staphylolyticus
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose resin column chromatography Staphylococcus simulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Staphylococcus aureus cell wall + H2O Staphylococcus aureus ATCC 6538 or ATCC 33807 cells Staphylococcus simulans ?
-
 ?

Synonyms

Synonyms Comment Organism
Lst
-
 Staphylococcus simulans