Cloned (Comment) | Organism |
---|---|
recominant expression of His-tagged lysostaphin by Escherichia coli BL21 (DE3) pEA3 | Staphylococcus simulans |
Protein Variants | Comment | Organism |
---|---|---|
D37A | no endopeptidase activity | Staphylococcus simulans |
H114A | no endopeptidase activity | Staphylococcus simulans |
H116A | no endopeptidase activity | Staphylococcus simulans |
H33A | no endopeptidase activity | Staphylococcus simulans |
H83A | no endopeptidase activity | Staphylococcus simulans |
T107A | reduced endopeptidase activity | Staphylococcus simulans |
Y81A | reduced endopeptidase activity | Staphylococcus simulans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16260 | - |
ESI-TOF MS, endopeptidase domain (residues 1-140) | Staphylococcus simulans |
28010 | - |
ESI-TOF MS, residues 247-493 | Staphylococcus simulans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus simulans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
zinc metal-chelate chromatography, cation-exchange chromatography | Staphylococcus simulans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
MV11 + H2O | developing a fluorescence resonance energy transfer substrate based on MV11 | Staphylococcus simulans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lysostaphin | - |
Staphylococcus simulans |