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Literature summary for 3.4.24.75 extracted from

  • Kumar, J.K.
    Lysostaphin: an antistaphylococcal agent (2008), Appl. Microbiol. Biotechnol., 80, 555-561.
    View publication on PubMed

Application

Application Comment Organism
analysis improvement of lipid extraction of staphylococcal cells Staphylococcus simulans
diagnostics detection of Staphylococcus aureus Staphylococcus simulans
food industry antistaphylococcal agent Staphylococcus simulans
medicine potential therapeutic agent against antibiotic-resistant Staphylococcus aureus infections Staphylococcus simulans
additional information antistaphylococcal agent in clinical laboratories Staphylococcus simulans

Cloned(Commentary)

Cloned (Comment) Organism
expression in Bacillus sphaericus Staphylococcus simulans

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+
-
Staphylococcus epidermidis
Zn2+
-
Staphylococcus simulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
-
Staphylococcus simulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Peptidoglycan + H2O Staphylococcus simulans activities of three enzymes namely, glycylglycine endopeptidase, endo-beta-N-acetyl glucosamidase and N-acteyl muramyl-L-alanine amidase. Lysostaphin rapidly lyses actively growing and non-dividing cells including staphylococci in biofilms. Glycylglycine endopeptidase lyses staphylococcal cells by hydrolyzing glycylglycine bonds in the polyglycine bridges which form cross links between glycopeptide chains in the cell wall peptidoglycan of Staphylococcus aureus cells. Lytic principle of lysostaphin is a peptidase which liberates N-terminal glycine and alanine from Staphylococcus aureus cell wall. Hexosaminidase is present in lysostaphin preparation is specific for the gluocainyl-muramic acid bond of the bacterial carbohydrate backbone Fragments of peptidoglycan
-
?

Organism

Organism UniProt Comment Textmining
Staphylococcus epidermidis
-
-
-
Staphylococcus simulans
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification lysostaphin is secreted as a proenzyme. Proteolytic cleavage of 13 N-terminal tandem repeats generates mature lysostaphin with 2 functionally separable domains Staphylococcus simulans

Purification (Commentary)

Purification (Comment) Organism
lysostaphin can be purified from Staphylococcus simulans strains Staphylococcus simulans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activities of three enzymes namely, glycylglycine endopeptidase, endo-beta-N-acetyl glucosamidase and N-acteyl muramyl-L-alanine amidase. Lysostaphin rapidly lyses actively growing and non-dividing cells including staphylococci in biofilms. Glycylglycine endopeptidase lyses staphylococcal cells by hydrolyzing glycylglycine bonds in the polyglycine bridges which form cross links between glycopeptide chains in the cell wall peptidoglycan of Staphylococcus aureus cells. Lytic principle of lysostaphin is a peptidase which liberates N-terminal glycine and alanine from Staphylococcus aureus cell wall. Hexosaminidase is present in lysostaphin preparation is specific for the gluocainyl-muramic acid bond of the bacterial carbohydrate backbone Staphylococcus simulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Peptidoglycan + H2O activities of three enzymes namely, glycylglycine endopeptidase, endo-beta-N-acetyl glucosamidase and N-acteyl muramyl-L-alanine amidase. Lysostaphin rapidly lyses actively growing and non-dividing cells including staphylococci in biofilms. Glycylglycine endopeptidase lyses staphylococcal cells by hydrolyzing glycylglycine bonds in the polyglycine bridges which form cross links between glycopeptide chains in the cell wall peptidoglycan of Staphylococcus aureus cells. Lytic principle of lysostaphin is a peptidase which liberates N-terminal glycine and alanine from Staphylococcus aureus cell wall. Hexosaminidase is present in lysostaphin preparation is specific for the gluocainyl-muramic acid bond of the bacterial carbohydrate backbone Staphylococcus simulans Fragments of peptidoglycan
-
?

Synonyms

Synonyms Comment Organism
lysostaphin
-
Staphylococcus simulans
r-lysostaphin recombinant lysostaphin Staphylococcus simulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5
-
half-life of the staphylolytic activity approximately 2 months Staphylococcus epidermidis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37
-
stable for 72 h, recombinant enzyme Staphylococcus simulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
half-life of the staphylolytic activity approximately 2 months Staphylococcus epidermidis