Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Staphylococcus simulans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 10 mM, 30% decrease of activity of enzyme produced with pET vector system and 20% decrease of activity of enzyme produced with pBAD vector system | Staphylococcus simulans | |
Cu2+ | 2 mM, 70-80% decrease of activity | Staphylococcus simulans | |
additional information | no inhibitory effect of EDTA and 2 mM Co2+ | Staphylococcus simulans | |
Ni2+ | 2 mM, 60% decrease of activity of enzyme produced with pET vector system and 20% decrease of activity of enzyme produced with pBAD vector system | Staphylococcus simulans | |
Zn2+ | 2 mM, 70-80% decrease of activity | Staphylococcus simulans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus simulans | P10547 | - |
- |
Purification (Comment) | Organism |
---|---|
cobalt Sepharose column chromatography | Staphylococcus simulans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
970 U/mg | Staphylococcus simulans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Peptidoglycan + H2O | - |
Staphylococcus simulans | Fragments of peptidoglycan | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Staphylococcus simulans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
enzymeproduced with pBAD vector system | Staphylococcus simulans |
8 | - |
enzyme obtained from pET vector system | Staphylococcus simulans |