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Literature summary for 3.4.24.75 extracted from
- Studies on prolysostaphin processing and characterization of lysostaphin immunity factor (Lif) of Staphylococcus simulans biovar staphylolyticus (1997), Mol. Microbiol., 23, 1251-1265.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli with pUC19 as vector | Staphylococcus simulans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibitory is trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane, i.e. E-64 | Staphylococcus simulans |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Staphylococcus simulans | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49120 | - |
prolysostaphin, calculation from nucleotide sequence. Prolysostaphin is processed to the mature enzyme by an extracellular cysteine protease | Staphylococcus simulans |
| 64000 | 68000 | the overestimated value is due to a high glutamate content. Prolysostaphin is processed to the mature enzyme by an extracellular cysteine protease | Staphylococcus simulans |
| 64000 | 68000 | SDS-PAGE of prolysostaphin | Staphylococcus simulans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus simulans | - |
biovar staphylolyticus, ATCC 1362 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Staphylococcus simulans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
169 U/mg (prolysostaphin), 760 U/mg (mature lysostaphin), 1 unit is defined as the amount of enzyme that causes 50% reduction of turbidity in 10 min at 37 C of an inactivated Staphylococcus carnosus TM300 cell suspension | Staphylococcus simulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Peptidoglycan + H2O | hydrolyis of pentaglycine interpeptide bridges of Staphylococcal peptidoglycan | Staphylococcus simulans | Fragments of peptidoglycan | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 64000-68000, prolysostaphin, the overestimated value is due to a high glutamate content | Staphylococcus simulans |
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