Literature summary for 3.4.24.72 extracted from

Liu, X.; Kopparapu, N.; Zheng, H.; Katrolia, P.; Deng, Y.; Zheng, X.
Purification and characterization of a fibrinolytic enzyme from the food-grade fungus, Neurospora sitophila (2016), J. Mol. Catal. B, 134, 98-104 .

No PubMed abstract available

Search for more literature for 3.4.24.72

Activating Compound

Activating Compound	Comment	Organism	Structure
2-mercaptoethanol	146% activity at 0.5% (v/v)	Neurospora sitophila
gelatin	121% activity at 1% (v/v)	Neurospora sitophila
glycerol	129% activity at 30% (v/v)	Neurospora sitophila

Inhibitors

Inhibitors	Comment	Organism	Structure
acetone	complete inhibition at 10% (v/v)	Neurospora sitophila
Aprotinin	82.3% residual activity at 5 mM	Neurospora sitophila
bovine serum albumin	complete inhibition at 1% (w/v)	Neurospora sitophila
Cu2+	61.2% residual activity at 5 mM	Neurospora sitophila
cysteine	47.4% residual activity at 20 mM	Neurospora sitophila
EDTA	13.5% residual activity at 5 mM	Neurospora sitophila
Fe3+	complete inhibition at 5 mM	Neurospora sitophila
L-lysine	38.5% residual activity at 5 mM	Neurospora sitophila
oxidized glutathione	complete inhibition at 5 mM	Neurospora sitophila
pepstatin	71.5% residual activity at 2.5 mM	Neurospora sitophila
peptone	61.5% residual activity at 1% (w/v)	Neurospora sitophila
phenylmethane sulfonyl fluoride	-	Neurospora sitophila
reduced glutathione	complete inhibition at 5 mM	Neurospora sitophila
SDS	complete inhibition at 0.1% (w/v)	Neurospora sitophila
Soybean trypsin inhibitor	complete inhibition at 2 mM	Neurospora sitophila
tosyl-phenylalanine chloromethyl ketone	76.2% residual activity at 5 mM	Neurospora sitophila
Urea	complete inhibition at 8 M	Neurospora sitophila

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	390% activity at 5 mM	Neurospora sitophila
Co2+	114% activity at 5 mM	Neurospora sitophila
Fe2+	106% activity at 5 mM	Neurospora sitophila
K+	144% activity at 5 mM	Neurospora sitophila
Mg2+	208% activity at 5 mM	Neurospora sitophila
Mn2+	168% activity at 5 mM	Neurospora sitophila
Na+	219% activity at 5 mM	Neurospora sitophila
Zn2+	122% activity at 5 mM	Neurospora sitophila

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
34000	-	gel filtration	Neurospora sitophila

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
fibrinogen + H2O	Neurospora sitophila	the alpha-chain of fibrinogen is degraded very quickly within 5 min, followed by beta-chain in 1 h whereas the gamma-chain is degraded in 2 h	fibrin + ?	-	?
plasminogen + H2O	Neurospora sitophila	-	plasmin + ?	-	?

Organism

Organism	UniProt	Comment	Textmining
Neurospora sitophila	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	-	Neurospora sitophila

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, octyl Sepharose column chromatography, SP Sepharose column chromatography, Sephadex G-25 gel filtration, and Superdex 75 gel filtration	Neurospora sitophila

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
fibrinogen + H2O	the alpha-chain of fibrinogen is degraded very quickly within 5 min, followed by beta-chain in 1 h whereas the gamma-chain is degraded in 2 h	Neurospora sitophila	fibrin + ?	-	?
plasminogen + H2O	-	Neurospora sitophila	plasmin + ?	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 34000, SDS-PAGE	Neurospora sitophila

Synonyms

Synonyms	Comment	Organism
fibrinolytic protease	-	Neurospora sitophila

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
41	-	-	Neurospora sitophila

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
23	46	more than 50% activity between 23 and 46°C	Neurospora sitophila

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
23	60	the enzyme is highly thermostable up to 52°C retaining more than 40% of its initial activity when incubated at 23-60°C for 1 h and 2 h	Neurospora sitophila

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.6	-	-	Neurospora sitophila

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.2	8.8	more than 60% activity between pH 5.2 and 8.8	Neurospora sitophila

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
5	11	the enzyme remains stable for 2 h at pH 5.0-11.0, retaining more than 80% activity	Neurospora sitophila

pI Value

Organism	Comment	pI Value Maximum	pI Value
Neurospora sitophila	isoelectric focusing	-	9.3

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)