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Literature summary for 3.4.24.72 extracted from

  • Khobragade, C.; Gophane, S.; Motwani, R.
    Purification and characterization of extracellular protease from soil isolate Stenotrophomonas maltophilia as novel target for fibrinolysis (2018), J. Microbiol. Biotechnol. Food Sci., 7, 496-502 .
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 5.0% residual activity at 5 mM Stenotrophomonas maltophilia
EDTA 6.25% residual activity at 5 mM Stenotrophomonas maltophilia
Hg2+ 17% residual activity at 5 mM Stenotrophomonas maltophilia
Zn2+ 11% residual activity at 5 mM Stenotrophomonas maltophilia

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Stenotrophomonas maltophilia
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 105% activity at 5 mM Stenotrophomonas maltophilia
K+ 114% activity at 5 mM Stenotrophomonas maltophilia
Mg2+ 112% activity at 5 mM Stenotrophomonas maltophilia
Na+ 112% activity at 5 mM Stenotrophomonas maltophilia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
gel filtration Stenotrophomonas maltophilia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
fibrinogen + H2O Stenotrophomonas maltophilia
-
fibrin + ?
-
?
fibrinogen + H2O Stenotrophomonas maltophilia CNK-7R
-
fibrin + ?
-
?

Organism

Organism UniProt Comment Textmining
Stenotrophomonas maltophilia
-
-
-
Stenotrophomonas maltophilia CNK-7R
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, dialysis and DEAE-cellulose column chromatography Stenotrophomonas maltophilia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
Stenotrophomonas maltophilia ?
-
?
casein + H2O
-
Stenotrophomonas maltophilia CNK-7R ?
-
?
fibrinogen + H2O
-
Stenotrophomonas maltophilia fibrin + ?
-
?
fibrinogen + H2O
-
Stenotrophomonas maltophilia CNK-7R fibrin + ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 47000, SDS-PAGE Stenotrophomonas maltophilia

Synonyms

Synonyms Comment Organism
fibrinolytic protease
-
Stenotrophomonas maltophilia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Stenotrophomonas maltophilia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Stenotrophomonas maltophilia