Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Aprotinin | strong inhibition | Bacillus subtilis | |
Ca2+ | strong inhibition | Bacillus subtilis | |
Co2+ | strong inhibition | Bacillus subtilis | |
Cu2+ | strong inhibition | Bacillus subtilis | |
EDTA | strong inhibition | Bacillus subtilis | |
EGTA | strong inhibition | Bacillus subtilis | |
Fe2+ | slight inhibition | Bacillus subtilis | |
Mg2+ | strong inhibition | Bacillus subtilis | |
Mn2+ | strong inhibition | Bacillus subtilis | |
Zn2+ | strong inhibition | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
x * 42000, the enzyme exists in three isoforms of 42000, 48000 and 60000 Da, SDS-PAGE | Bacillus subtilis |
48000 | - |
x * 48000, the enzyme exists in three isoforms of 42000, 48000 and 60000 Da, SDS-PAGE | Bacillus subtilis |
60000 | - |
x * 60000, the enzyme exists in three isoforms of 42000, 48000 and 60000 Da, SDS-PAGE | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
fibrinogen + H2O | Bacillus subtilis | - |
fibrin + ? | - |
? | |
fibrinogen + H2O | Bacillus subtilis I-2 | - |
fibrin + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bacillus subtilis I-2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and DEAE-Sephadex gel filtration | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
fibrinogen + H2O | - |
Bacillus subtilis | fibrin + ? | - |
? | |
fibrinogen + H2O | - |
Bacillus subtilis I-2 | fibrin + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, the enzyme exists in three isoforms of 42000, 48000 and 60000 Da, SDS-PAGE | Bacillus subtilis |
? | x * 48000, the enzyme exists in three isoforms of 42000, 48000 and 60000 Da, SDS-PAGE | Bacillus subtilis |
? | x * 60000, the enzyme exists in three isoforms of 42000, 48000 and 60000 Da, SDS-PAGE | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
fibrinolytic protease | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | considerable activity is observed at 60°C (88.6%) and at 40°C (68.6%) | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | the enzyme is quite stable at 30-50°C for 60 min, but at 60°C and above activity decreases drastically | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | considerable activity is observed at pH 10.0 (84.4%). The enzyme exhibits poorer activity in the acidic pH 5.0-6.0 (53-64%) | Bacillus subtilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | after 60 min incubation the enzyme possesses remarkable stability at pH 7.0-10.0 (98.5-100%). In the acidic pH (5.0-6.0), the enzyme retains 77 and 85% activity, respectively | Bacillus subtilis |
Organism | Comment | Expression |
---|---|---|
Bacillus subtilis | soybean meal supports maximum protease production, followed by malt extract, cotton cake, gelatin and beef extract | up |