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Literature summary for 3.4.24.72 extracted from
- Starase: A bi-functional fibrinolytic protease from hepatic caeca of Asterina pectinifera displays antithrombotic potential (2014), Biochimie, 105, 45-57.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | starase has the potential to be a potent thrombolytic agent due to its bi-functional properties (containing both direct-acting and plasminogen-activating activities) and lack of hemorrhagic activity | Patiria pectinifera |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-amidinophenylmethylsulfonyl fluoride | 5% residual activity at 2 mM | Patiria pectinifera |  |
| additional information | not inhibited by EDTA and EGTA | Patiria pectinifera | |
| phenylmethylsulfonyl fluoride | 4% residual activity at 2 mM | Patiria pectinifera | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.37 | - |
D-Val-Leu-Lys-4-nitroanilide | at pH 7.4 and 37°C | Patiria pectinifera | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme activity is not influenced by Mn2+, Cu2+, Ca2+, Mg2+, Co2+, Zn2+, and Fe2+ | Patiria pectinifera |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
1 * 48000, SDS-PAGE | Patiria pectinifera |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| fibrinogen + H2O | Patiria pectinifera | the alpha-chain is rapidly hydrolyzed and the beta-chain is partially digested by 10 ng of starase. With an increase in starase concentration to 120 ng, the gamma-chain is partially degraded, but all the alpha-, beta- and gamma-chains of fibrinogen are completely cleaved by 250 ng and higher concentration of starase | fibrin + ? | - |
? | |
| plasminogen + H2O | Patiria pectinifera | - |
plasmin + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Patiria pectinifera | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Sepharose CL6B column chromatography, Sepharose CL4B column chromatography, and Mono Q HR column chromatography | Patiria pectinifera |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hepatic cecum | - |
Patiria pectinifera | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 20 mM Tris-HCl buffer, 3 months, 3% loss of enzyme activity | Patiria pectinifera |
| 4°C, 20 mM Tris-HCl buffer, 3 months, 5% loss of enzyme activity | Patiria pectinifera |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| bovine albumin + H2O | - |
Patiria pectinifera | ? | - |
? | |
| bovine gamma-globulin + H2O | - |
Patiria pectinifera | ? | - |
? | |
| D-Val-Leu-Lys-4-nitroanilide + H2O | highest activity | Patiria pectinifera | D-Val-Leu-Lys + 4-nitroaniline | - |
? | |
| extracellular matrix component type VII collagen | - |
Patiria pectinifera | ? | - |
? | |
| factor Xa + H2O | - |
Patiria pectinifera | ? | - |
? | |
| fibrinogen + H2O | the alpha-chain is rapidly hydrolyzed and the beta-chain is partially digested by 10 ng of starase. With an increase in starase concentration to 120 ng, the gamma-chain is partially degraded, but all the alpha-, beta- and gamma-chains of fibrinogen are completely cleaved by 250 ng and higher concentration of starase | Patiria pectinifera | fibrin + ? | - |
? | |
| additional information | the enzyme shows weak amidolytic activity for the substrates Z-D-Arg-Gly-Arg-4-nitroanilide and D-Phe-Pip-Arg-4-nitroanilide | Patiria pectinifera | ? | - |
? | |
| plasminogen + H2O | - |
Patiria pectinifera | plasmin + ? | - |
? | |
| pyroGlu-Gly-Arg-4-nitroanilide + H2O | - |
Patiria pectinifera | pyroGlu-Gly-Arg + 4-nitroaniline | - |
? | |
| thrombin + H2O | - |
Patiria pectinifera | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 48000, SDS-PAGE | Patiria pectinifera |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| fibrinolytic protease | - |
Patiria pectinifera |
| starase | - |
Patiria pectinifera |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Patiria pectinifera |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Patiria pectinifera |
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