Activating Compound | Comment | Organism | Structure |
---|---|---|---|
captopril | solECE, activity 106.9% | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
solECE-1 constructed by fusing the cleavable N-terminalsignal sequence of human alkaline phosphatase in frame with the entire extracellular domain of ECE-1, transfected into CHO-K1 cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Homo sapiens | |
EDTA | - |
Homo sapiens | |
additional information | little or no inhibition with captopril | Homo sapiens | |
PD 069185 | - |
Homo sapiens | |
phosphoramidon | - |
Homo sapiens | |
thiorphan | 50% inhibition of native ECE-1, 70% inhibition of solECE-1 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0027 | - |
Big endothelin-1 | pH 6.8, 37°C, ECE-1a | Homo sapiens | |
0.00465 | - |
Big endothelin-1 | pH 6.4, 37°C, solECE-1 | Homo sapiens | |
0.00751 | - |
Big endothelin-1 | pH 6.4, 37°C, ECE-1a | Homo sapiens | |
0.0082 | - |
Big endothelin-1 | pH 6.8, 37°C, solECE-1a | Homo sapiens | |
0.01987 | - |
Big endothelin-1 | pH 7.1, 37°C, ECE-1a | Homo sapiens | |
0.04831 | - |
Big endothelin-1 | pH 7.1, 37°C, solECE-1a | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
107600 | - |
2 * 110000, SDS-PAGE, 2 * 107600, MALDI | Homo sapiens |
110000 | - |
2 * 110000, SDS-PAGE, 2 * 107600, MALDI | Homo sapiens |
232000 | - |
gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
big endothelin-1 + H2O | Homo sapiens | key enzyme in the biosynthesis of the endothelins | endothelin + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
human | - |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0001799 | - |
recombinant enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
big endothelin-1 + H2O | - |
Homo sapiens | endothelin + ? | - |
? | |
big endothelin-1 + H2O | key enzyme in the biosynthesis of the endothelins | Homo sapiens | endothelin + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 110000, SDS-PAGE, 2 * 107600, MALDI | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
ECE-1 | - |
Homo sapiens |
endothelin-converting enzyme-1 | - |
Homo sapiens |
solECE-1 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00528 | - |
Big endothelin-1 | pH 6.4, 37°C, ECE-1a | Homo sapiens | |
0.097 | - |
Big endothelin-1 | pH 6.4, 37°C, solECE-1 | Homo sapiens | |
0.112 | - |
Big endothelin-1 | pH 6.8, 37°C, ECE-1a | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.1 | 6.4 | solECE-1 | Homo sapiens |
6.7 | 6.9 | ECE-1 | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6.8 | solECE-1 half-maximal activity at pH 5.7 and pH 6.7 | Homo sapiens |
5.7 | 7.3 | native ECE-1, half-maximal activity at pH 6.2 and pH 7.0 | Homo sapiens |