Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.71 extracted from

  • Ahn, K.; Herman, S.B.; Fahnoe, D.C.
    Soluble human endothelin-converting enzyme-1: expression, purification, and demonstration of pronounced pH sensitivity (1998), Arch. Biochem. Biophys., 359, 258-268.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
captopril solECE, activity 106.9% Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
solECE-1 constructed by fusing the cleavable N-terminalsignal sequence of human alkaline phosphatase in frame with the entire extracellular domain of ECE-1, transfected into CHO-K1 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Homo sapiens
EDTA
-
Homo sapiens
additional information little or no inhibition with captopril Homo sapiens
PD 069185
-
Homo sapiens
phosphoramidon
-
Homo sapiens
thiorphan 50% inhibition of native ECE-1, 70% inhibition of solECE-1 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0027
-
Big endothelin-1 pH 6.8, 37°C, ECE-1a Homo sapiens
0.00465
-
Big endothelin-1 pH 6.4, 37°C, solECE-1 Homo sapiens
0.00751
-
Big endothelin-1 pH 6.4, 37°C, ECE-1a Homo sapiens
0.0082
-
Big endothelin-1 pH 6.8, 37°C, solECE-1a Homo sapiens
0.01987
-
Big endothelin-1 pH 7.1, 37°C, ECE-1a Homo sapiens
0.04831
-
Big endothelin-1 pH 7.1, 37°C, solECE-1a Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
107600
-
2 * 110000, SDS-PAGE, 2 * 107600, MALDI Homo sapiens
110000
-
2 * 110000, SDS-PAGE, 2 * 107600, MALDI Homo sapiens
232000
-
gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
big endothelin-1 + H2O Homo sapiens key enzyme in the biosynthesis of the endothelins endothelin + ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
human
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0001799
-
recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
big endothelin-1 + H2O
-
Homo sapiens endothelin + ?
-
?
big endothelin-1 + H2O key enzyme in the biosynthesis of the endothelins Homo sapiens endothelin + ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 110000, SDS-PAGE, 2 * 107600, MALDI Homo sapiens

Synonyms

Synonyms Comment Organism
ECE-1
-
Homo sapiens
endothelin-converting enzyme-1
-
Homo sapiens
solECE-1
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00528
-
Big endothelin-1 pH 6.4, 37°C, ECE-1a Homo sapiens
0.097
-
Big endothelin-1 pH 6.4, 37°C, solECE-1 Homo sapiens
0.112
-
Big endothelin-1 pH 6.8, 37°C, ECE-1a Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1 6.4 solECE-1 Homo sapiens
6.7 6.9 ECE-1 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
5.5 6.8 solECE-1 half-maximal activity at pH 5.7 and pH 6.7 Homo sapiens
5.7 7.3 native ECE-1, half-maximal activity at pH 6.2 and pH 7.0 Homo sapiens