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Literature summary for 3.4.24.7 extracted from

  • Knauper, V.; Patterson, M.L.; Gomis-Ruth, F.X.; Smith, B.; Lyons, A.; Docherty, A.J.; Murphy, G.
    The role of exon 5 in fibroblast collagenase (MMP-1) substrate specificity and inhibitor selectivity (2001), Eur. J. Biochem., 268, 1888-1896.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information construction of a chimeric enzyme, the exon 5 chimera, consisting primarily of MMP-1, with the region coded for by exon 5 replaced with the equivalent region of MMP-3, a noncollagenolytic MMP. Unlike MMP-3, the exon 5 chimera is capable of cleaving type I collagen, but the activity is only 2.2% of the trypsin-activated MMP-1. The kinetics for exon 5 chimera cleavage of two synthetic substrates display an MMP-3 phenotype, however, cleavage of gelatin is slightly impaired as compared to the parent enzymes. The KI-values for the exon 5 chimera complexed with synthetic inhibitors and N-terminal TIMP-2 show a more MMP-3-like behaviour. The exon 5 mutant shows a 2.9fold in the ratio of turnover number to Km-value with (7-methoxycoumarin-4-yl)-acetyl-Pro-Leu-Gly-Leu-(3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl)-Ala-Arg-NH2 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
CT 1746
-
Homo sapiens
Ro-31-9790
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
x * 50000, exon 5 mutant species, SDS-PAGE Homo sapiens
58000
-
x * 58000, N-glycosylated exon 5 mutant species, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and exon 5 mutant enzyme Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
fibroblast
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(7-methoxycoumarin-4-yl)-acetyl-Pro-Leu-Gly-Leu-(3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl)-Ala-Arg-NH2 + H2O
-
Homo sapiens (7-methoxycoumarin-4-yl)-acetyl-Pro-Leu-Gly + Leu-(3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl)-Ala-Arg-NH2
-
?
Collagen + H2O type I collagen Homo sapiens ?
-
?
Gelatin + H2O
-
Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
? x * 50000, exon 5 mutant species, SDS-PAGE Homo sapiens
? x * 58000, N-glycosylated exon 5 mutant species, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
MMP-1
-
Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000108
-
Ro31-9790 wild-type enzyme Homo sapiens
0.0000128
-
CT 1746 exon 5 mutant enzyme Homo sapiens
0.0000603
-
CT 1746 wild-type enzyme Homo sapiens
0.0001893
-
Ro31-9790 exon 5 mutant enzyme Homo sapiens