Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a chimeric enzyme, the exon 5 chimera, consisting primarily of MMP-1, with the region coded for by exon 5 replaced with the equivalent region of MMP-3, a noncollagenolytic MMP. Unlike MMP-3, the exon 5 chimera is capable of cleaving type I collagen, but the activity is only 2.2% of the trypsin-activated MMP-1. The kinetics for exon 5 chimera cleavage of two synthetic substrates display an MMP-3 phenotype, however, cleavage of gelatin is slightly impaired as compared to the parent enzymes. The KI-values for the exon 5 chimera complexed with synthetic inhibitors and N-terminal TIMP-2 show a more MMP-3-like behaviour. The exon 5 mutant shows a 2.9fold in the ratio of turnover number to Km-value with (7-methoxycoumarin-4-yl)-acetyl-Pro-Leu-Gly-Leu-(3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl)-Ala-Arg-NH2 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CT 1746 | - |
Homo sapiens | |
Ro-31-9790 | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, exon 5 mutant species, SDS-PAGE | Homo sapiens |
58000 | - |
x * 58000, N-glycosylated exon 5 mutant species, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and exon 5 mutant enzyme | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fibroblast | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(7-methoxycoumarin-4-yl)-acetyl-Pro-Leu-Gly-Leu-(3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl)-Ala-Arg-NH2 + H2O | - |
Homo sapiens | (7-methoxycoumarin-4-yl)-acetyl-Pro-Leu-Gly + Leu-(3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl)-Ala-Arg-NH2 | - |
? | |
Collagen + H2O | type I collagen | Homo sapiens | ? | - |
? | |
Gelatin + H2O | - |
Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, exon 5 mutant species, SDS-PAGE | Homo sapiens |
? | x * 58000, N-glycosylated exon 5 mutant species, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
MMP-1 | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000108 | - |
Ro31-9790 | wild-type enzyme | Homo sapiens | |
0.0000128 | - |
CT 1746 | exon 5 mutant enzyme | Homo sapiens | |
0.0000603 | - |
CT 1746 | wild-type enzyme | Homo sapiens | |
0.0001893 | - |
Ro31-9790 | exon 5 mutant enzyme | Homo sapiens |