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Literature summary for 3.4.24.69 extracted from
- Mechanism of substrate recognition by the novel botulinum neurotoxin subtype F5 (2016), Sci. Rep., 6, 19875 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Clostridium botulinum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D161A | the mutant exhibits a 1000fold reduction of wild type activity | Clostridium botulinum |
| D70A | the mutant exhibits a 10fold reduction of wild type activity | Clostridium botulinum |
| D70A/D161A | inactive | Clostridium botulinum |
| E147A/E308A | the mutant exhibits a 5fold reduction of wild type activity | Clostridium botulinum |
| E147R | the mutant exhibits a 80fold reduction of wild type activity | Clostridium botulinum |
| E308R | the mutant exhibits a 0.8fold reduction of wild type activity | Clostridium botulinum |
| K218A | inactive | Clostridium botulinum |
| K218D | inactive | Clostridium botulinum |
| K218R | the mutant exhibits an 8fold reduction of wild type activity | Clostridium botulinum |
| K58A | the mutant exhibits a 250fold reduction of wild type activity | Clostridium botulinum |
| R133A | the mutant exhibits a 300fold reduction of wild type activity | Clostridium botulinum |
| T192A | the mutant exhibits a 3fold reduction of wild type activity | Clostridium botulinum |
| Y183A | the mutant exhibits a 3.2fold reduction of wild type activity | Clostridium botulinum |
| Y183A/Y239A | the mutant exhibits a 1000fold reduction of wild type activity | Clostridium botulinum |
| Y239A | the mutant exhibits an 8fold reduction of wild type activity | Clostridium botulinum |
| Y26A | the mutant exhibits a 60fold reduction of wild type activity | Clostridium botulinum |
| Y26A/Y50A/T192A | the mutant exhibits a 600fold reduction of wild type activity | Clostridium botulinum |
| Y50A | the mutant exhibits a 10fold reduction of wild type activity | Clostridium botulinum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00513 | - |
vesicle-associated membrane protein-2 | wild type enzyme, pH and temperature not specified in the publication | Clostridium botulinum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| vesicle-associated membrane protein-2 + H2O | Clostridium botulinum | cleavage at Leu54-Glu55 | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium botulinum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Clostridium botulinum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| vesicle-associated membrane protein-2 + H2O | cleavage at Leu54-Glu55 | Clostridium botulinum | ? | - |
? | |
| vesicle-associated membrane protein-2 mutant D51A + H2O | the enzyme cleaves the mutant substrate with about 10fold lower efficiency compared to the wild type substrate | Clostridium botulinum | ? | - |
? | |
| vesicle-associated membrane protein-2 mutant E41A + H2O | the enzyme cleaves the mutant substrate with about 80fold lower efficiency compared to the wild type substrate | Clostridium botulinum | ? | - |
? | |
| vesicle-associated membrane protein-2 mutant E55A + H2O | the enzyme cleaves the mutant substrate with about 750fold lower efficiency compared to the wild type substrate | Clostridium botulinum | ? | - |
? | |
| vesicle-associated membrane protein-2 mutant K52A + H2O | the enzyme cleaves the mutant substrate with about 20fold lower efficiency compared to the wild type substrate | Clostridium botulinum | ? | - |
? | |
| vesicle-associated membrane protein-2 mutant N49A + H2O | the enzyme cleaves the mutant substrate with about 10fold lower efficiency compared to the wild type substrate | Clostridium botulinum | ? | - |
? | |
| vesicle-associated membrane protein-2 mutant Q58A + H2O | the enzyme cleaves the mutant substrate with about 25fold lower efficiency compared to the wild type substrate | Clostridium botulinum | ? | - |
? | |
| vesicle-associated membrane protein-2 mutant R31A + H2O | the enzyme cleaves the mutant substrate with about 20fold lower efficiency compared to the wild type substrate | Clostridium botulinum | ? | - |
? | |
| vesicle-associated membrane protein-2 mutant R56A + H2O | the enzyme cleaves the mutant substrate with about 4000fold lower efficiency compared to the wild type substrate | Clostridium botulinum | ? | - |
? | |
| vesicle-associated membrane protein-2 mutant V50A + H2O | the enzyme cleaves the mutant substrate with about 50fold lower efficiency compared to the wild type substrate | Clostridium botulinum | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BoNT/F5 | - |
Clostridium botulinum |
| botulinum neurotoxin subtype F5 | - |
Clostridium botulinum |
| LC/F5 | catalytic domain of botulinum neurotoxin subtype F5 | Clostridium botulinum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 938.9 | - |
vesicle-associated membrane protein-2 | wild type enzyme, pH and temperature not specified in the publication | Clostridium botulinum |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 183020 | - |
vesicle-associated membrane protein-2 | wild type enzyme, pH and temperature not specified in the publication | Clostridium botulinum |
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Release 2023.1 (January 2023)
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