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Literature summary for 3.4.24.69 extracted from

  • Mizanur, R.; Frasca, V.; Swaminathan, S.; Bavari, S.; Webb, R.; Smith, L.; Ahmed, S.
    The C terminus of the catalytic domain of type A botulinum neurotoxin may facilitate product release from the active site (2013), J. Biol. Chem., 288, 24223-24233.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.69

Protein Variants

Protein Variants Comment Organism
R230L inactive Clostridium botulinum
Y365N inactive Clostridium botulinum

Inhibitors

Inhibitors Comment Organism Structure
24-mer C-terminal peptide of LcE1 the activity of the light chain of botulinum toxin A is significantly reduced to 32% by the peptide with sequence TGRGLVKKIIRFCKNIVSVKGIRK Clostridium botulinum
32-mer C-terminal peptide of LcA the activity of the light chain of botulinum toxin A is significantly reduced to 15% by the peptide with sequence KNFTGLFEFYKLLCVRGIITSKTKSLDKGYNK Clostridium botulinum
additional information the C-terminal peptides of LcB-1, LcC1-1, LcD-1, and LcF-1 show no inhibitory effect Clostridium botulinum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6
-
 SNAP-25 full-length light chain of botulinum toxin A, in 50 mM HEPES, pH 7.4, at 37°C Clostridium botulinum

Organism

Organism UniProt Comment Textmining
Clostridium botulinum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
SNAP-25 + H2O
-
 Clostridium botulinum ?
-
 ?
VAMP + H2O
-
 Clostridium botulinum ?
-
 ?

Synonyms

Synonyms Comment Organism
BoNT/A
-
 Clostridium botulinum
Botulinum neurotoxin
-
 Clostridium botulinum
LCA light chain of botulinum neurotoxin A Clostridium botulinum
LcB light chain of botulinum neurotoxin B Clostridium botulinum
lcc1 light chain of botulinum neurotoxin C1 Clostridium botulinum
LCD light chain of botulinum neurotoxin D Clostridium botulinum
LCE light chain of botulinum neurotoxin E Clostridium botulinum
LCF light chain of botulinum neurotoxin F Clostridium botulinum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
38.1
-
 SNAP-25 full-length light chain of botulinum toxin A, in 50 mM HEPES, pH 7.4, at 37°C Clostridium botulinum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00453
-
 32-mer C-terminal peptide of LcA full-length light chain of botulinum toxin A, in 50 mM HEPES, pH 7.4, at 37°C Clostridium botulinum