Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Clostridium botulinum |
Protein Variants | Comment | Organism |
---|---|---|
D877N | the mutant shows 20% reduced neurotoxicity compared to the wild type | Clostridium botulinum |
E48Q | the mutant shows 64% reduced neurotoxicity compared to the wild type | Clostridium botulinum |
E48Q/D877N | the mutant shows 8% increased neurotoxicity due to faster cytosolic delivery of the enzymatic domain | Clostridium botulinum |
E48Q/E653K | the mutant shows 9% reduced neurotoxicity compared to the wild type | Clostridium botulinum |
E48Q/E653Q | the mutant shows 38% reduced neurotoxicity compared to the wild type | Clostridium botulinum |
E48Q/E653Q/D877N | the mutant shows 54% increased neurotoxicity due to faster cytosolic delivery of the enzymatic domain | Clostridium botulinum |
E653 K/D877N | the mutant shows 28% increased neurotoxicity due to faster cytosolic delivery of the enzymatic domain | Clostridium botulinum |
E653K | the mutant shows 15% reduced neurotoxicity compared to the wild type | Clostridium botulinum |
E653Q | the mutant shows 31% reduced neurotoxicity compared to the wild type | Clostridium botulinum |
E653Q/D877N | the mutant shows 32% increased neurotoxicity due to faster cytosolic delivery of the enzymatic domain | Clostridium botulinum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
SNAP-25 + H2O | Clostridium botulinum | - |
? | - |
? | |
SNAP-25 + H2O | Clostridium botulinum Okra | - |
? | - |
? | |
synaptobrevin + H2O | Clostridium botulinum | - |
? | - |
? | |
synaptobrevin + H2O | Clostridium botulinum Okra | - |
? | - |
? | |
VAMP-2 + H2O | Clostridium botulinum | - |
? | - |
? | |
VAMP-2 + H2O | Clostridium botulinum Okra | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium botulinum | P10844 | - |
- |
Clostridium botulinum Okra | P10844 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Clostridium botulinum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
SNAP-25 + H2O | - |
Clostridium botulinum | ? | - |
? | |
SNAP-25 + H2O | - |
Clostridium botulinum Okra | ? | - |
? | |
synaptobrevin + H2O | - |
Clostridium botulinum | ? | - |
? | |
synaptobrevin + H2O | - |
Clostridium botulinum Okra | ? | - |
? | |
VAMP-2 + H2O | - |
Clostridium botulinum | ? | - |
? | |
VAMP-2 + H2O | - |
Clostridium botulinum Okra | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BoNT/B | serotype | Clostridium botulinum |
Botulinum neurotoxin | - |
Clostridium botulinum |