Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.69 extracted from

  • Pirazzini, M.; Henke, T.; Rossetto, O.; Mahrhold, S.; Krez, N.; Rummel, A.; Montecucco, C.; Binz, T.
    Neutralisation of specific surface carboxylates speeds up translocation of botulinum neurotoxin type B enzymatic domain (2013), FEBS Lett., 587, 3831-3836.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Clostridium botulinum

Protein Variants

Protein Variants Comment Organism
D877N the mutant shows 20% reduced neurotoxicity compared to the wild type Clostridium botulinum
E48Q the mutant shows 64% reduced neurotoxicity compared to the wild type Clostridium botulinum
E48Q/D877N the mutant shows 8% increased neurotoxicity due to faster cytosolic delivery of the enzymatic domain Clostridium botulinum
E48Q/E653K the mutant shows 9% reduced neurotoxicity compared to the wild type Clostridium botulinum
E48Q/E653Q the mutant shows 38% reduced neurotoxicity compared to the wild type Clostridium botulinum
E48Q/E653Q/D877N the mutant shows 54% increased neurotoxicity due to faster cytosolic delivery of the enzymatic domain Clostridium botulinum
E653 K/D877N the mutant shows 28% increased neurotoxicity due to faster cytosolic delivery of the enzymatic domain Clostridium botulinum
E653K the mutant shows 15% reduced neurotoxicity compared to the wild type Clostridium botulinum
E653Q the mutant shows 31% reduced neurotoxicity compared to the wild type Clostridium botulinum
E653Q/D877N the mutant shows 32% increased neurotoxicity due to faster cytosolic delivery of the enzymatic domain Clostridium botulinum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
SNAP-25 + H2O Clostridium botulinum
-
?
-
?
SNAP-25 + H2O Clostridium botulinum Okra
-
?
-
?
synaptobrevin + H2O Clostridium botulinum
-
?
-
?
synaptobrevin + H2O Clostridium botulinum Okra
-
?
-
?
VAMP-2 + H2O Clostridium botulinum
-
?
-
?
VAMP-2 + H2O Clostridium botulinum Okra
-
?
-
?

Organism

Organism UniProt Comment Textmining
Clostridium botulinum P10844
-
-
Clostridium botulinum Okra P10844
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Clostridium botulinum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
SNAP-25 + H2O
-
Clostridium botulinum ?
-
?
SNAP-25 + H2O
-
Clostridium botulinum Okra ?
-
?
synaptobrevin + H2O
-
Clostridium botulinum ?
-
?
synaptobrevin + H2O
-
Clostridium botulinum Okra ?
-
?
VAMP-2 + H2O
-
Clostridium botulinum ?
-
?
VAMP-2 + H2O
-
Clostridium botulinum Okra ?
-
?

Synonyms

Synonyms Comment Organism
BoNT/B serotype Clostridium botulinum
Botulinum neurotoxin
-
Clostridium botulinum