Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | required | Clostridium botulinum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Clostridium botulinum | the catalytic light chains of BoNTs, BoNT-LC, recognize extended regions of their substrates for cleavage | ? | - |
? | |
SNAP-25 + H2O | Clostridium botulinum | i.e. 25 kDa synaptosome-associated protein, BoNT/A requires two extended exosites for optimal substrate binding and recognition of its intracellular target SNAP-25 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium botulinum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the catalytic light chains of BoNTs, BoNT-LC, recognize extended regions of their substrates for cleavage | Clostridium botulinum | ? | - |
? | |
additional information | feasibility of using the CFP-YFP pair with full-length SNAP-25 as a FRET-based substrate for BoNT/A in a cell-based assay or with the 66-mer peptide as a FRET substrate in an in vitro assay, optimization of FRET efficiency by use of fluorescent protein variants, CsY, CsYY or YsCsY, overview | Clostridium botulinum | ? | - |
? | |
SNAP-25 + H2O | i.e. 25 kDa synaptosome-associated protein, BoNT/A requires two extended exosites for optimal substrate binding and recognition of its intracellular target SNAP-25 | Clostridium botulinum | ? | - |
? | |
SNAP-25 peptide (141-206) + H2O | the minimal size of SNAP-25 known to retain full activity as a BoNT/A substrate is the C-terminal 66-mer peptide, residues 141-206, with both exosites | Clostridium botulinum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BoNT/A | - |
Clostridium botulinum |
Botulinum neurotoxin | - |
Clostridium botulinum |
botulinum neurotoxin a | - |
Clostridium botulinum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Clostridium botulinum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Clostridium botulinum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the non-fluorescent substrate GST-SNAP-25(141-206) is a competitive inhibitor in the fluorometric assay for CsY and YsCsY with Ki values of 0.0076 and 0.0045 mM, respectively | Clostridium botulinum |