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Literature summary for 3.4.24.69 extracted from

  • Chen, S.; Barbieri, J.T.
    Engineering botulinum neurotoxin to extend therapeutic intervention (2009), Proc. Natl. Acad. Sci. USA, 106, 9180-9184.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.69

Protein Variants

Protein Variants Comment Organism
K224D mutation of BoNT/E light chain. The mutant shows extended substrate specificity to cleave SNAP-23, and the natural substrate, SNAP-25, but not SNAP-29 or SNAP-47, introduction into HeLa cells Clostridium botulinum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
SNAP-25 + H2O Clostridium botulinum a neuronal SNARE protein ?
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 ?

Organism

Organism UniProt Comment Textmining
Clostridium botulinum
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information BoNT/E light chain mutant K224D does not cleave the SNARE proteins SNAP29 or SNAP47 Clostridium botulinum ?
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 ?
SNAP-23 + H2O a nonneuronal SNARE protein, that mediates vesicle-plasma membrane fusion processes, including secretion of airway mucus, antibody, insulin, gastric acids, and ions. SNAP23 is cleaved by an engineered BoNT/E light chain, LC/E K224D. Molecular modeling of the enzyme-substrate complex using the crystal structure of LC/E, Protein Data Bank ID 3d3x, overview Clostridium botulinum ?
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 ?
SNAP-25 + H2O a neuronal SNARE protein Clostridium botulinum ?
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 ?
SNAP-25 + H2O a neuronal SNARE protein, cleaved by an engineered BoNT/E light chain, LC/E K224D Clostridium botulinum ?
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 ?

Synonyms

Synonyms Comment Organism
Botulinum neurotoxin
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 Clostridium botulinum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Clostridium botulinum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Clostridium botulinum

General Information

General Information Comment Organism
physiological function Clostridium botulinum neurotoxins are effective therapeutics for a variety of neurological disorders, such as strabismus, blepharospam, hemificial spasm, and cervical dystonia, because of the toxin's tropism for neurons and specific cleavage of neuronal soluble N-ethylmaleimide-sensitive fusion protein-attachment protein receptors, SNARE, proteins Clostridium botulinum