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Literature summary for 3.4.24.69 extracted from

  • Scotcher, M.C.; McGarvey, J.A.; Johnson, E.A.; Stanker, L.H.
    Epitope characterization and variable region sequence of f1-40, a high-affinity monoclonal antibody to botulinum neurotoxin type a (Hall strain) (2009), PLoS ONE, 4, e4924.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
heavy and light chain DNA sequence determination, expression of GST-tagged wild-type BoNT/A light chain and of seven peptide fragments of BoNT/A light chain, Lc, L1, L2, L1-1, L1-2, L1-3, L1-4, as well as mutant L1-3 BoNT/A light chain peptide fragment in Escherichia coli strain Bl21(DE3) Clostridium botulinum

Protein Variants

Protein Variants Comment Organism
Q138G/P139G/D140G site-directed mutagenesis, the mutant is no longer recognized by antibody F1-40 Clostridium botulinum

Inhibitors

Inhibitors Comment Organism Structure
antibody F1-40 F1-40 binds a peptide fragment of the BoNT/A light chain, designated L1-3, which spans from T125 to L200, with recognition motif QPDRS. No binding to BoNT/A mutant Q138G/P139G/D140G. Wild-type residues Q138, P139 and D140 form a loop on the external surface of BoNT/A, exposed to solvent Clostridium botulinum

Organism

Organism UniProt Comment Textmining
Clostridium botulinum
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information F1-40 is a mouse-derived, IgG1 monoclonal antibody that binds the light chain of BoNT serotype A and is used in a sensitive immunoassay for toxin detection, determination of binding epitopes, overview Clostridium botulinum ?
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Subunits

Subunits Comment Organism
More BoNT is a 150 kDa protein consisting of a 100 kDa heavy-chain, Hc, and a 50 kDa light-chain, Lc. Epitope mapping of the light chain, overview Clostridium botulinum

Synonyms

Synonyms Comment Organism
BoNT serotype A
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Clostridium botulinum
BoNT/A
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Clostridium botulinum