Cloned (Comment) | Organism |
---|---|
heavy and light chain DNA sequence determination, expression of GST-tagged wild-type BoNT/A light chain and of seven peptide fragments of BoNT/A light chain, Lc, L1, L2, L1-1, L1-2, L1-3, L1-4, as well as mutant L1-3 BoNT/A light chain peptide fragment in Escherichia coli strain Bl21(DE3) | Clostridium botulinum |
Protein Variants | Comment | Organism |
---|---|---|
Q138G/P139G/D140G | site-directed mutagenesis, the mutant is no longer recognized by antibody F1-40 | Clostridium botulinum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
antibody F1-40 | F1-40 binds a peptide fragment of the BoNT/A light chain, designated L1-3, which spans from T125 to L200, with recognition motif QPDRS. No binding to BoNT/A mutant Q138G/P139G/D140G. Wild-type residues Q138, P139 and D140 form a loop on the external surface of BoNT/A, exposed to solvent | Clostridium botulinum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium botulinum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | F1-40 is a mouse-derived, IgG1 monoclonal antibody that binds the light chain of BoNT serotype A and is used in a sensitive immunoassay for toxin detection, determination of binding epitopes, overview | Clostridium botulinum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | BoNT is a 150 kDa protein consisting of a 100 kDa heavy-chain, Hc, and a 50 kDa light-chain, Lc. Epitope mapping of the light chain, overview | Clostridium botulinum |
Synonyms | Comment | Organism |
---|---|---|
BoNT serotype A | - |
Clostridium botulinum |
BoNT/A | - |
Clostridium botulinum |