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Literature summary for 3.4.24.69 extracted from

  • Pellett, S.; Tepp, W.H.; Toth, S.I.; Johnson, E.A.
    Comparison of the primary rat spinal cord cell (RSC) assay and the mouse bioassay for botulinum neurotoxin type A potency determination (2010), J. Pharmacol. Toxicol. Methods, 61, 304-310.
    View publication on PubMed
Search for more literature for 3.4.24.69

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
SNAP-25 + H2O Clostridium botulinum i.e. 25-kDa synaptosomal-associated protein ?
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 ?

Organism

Organism UniProt Comment Textmining
Clostridium botulinum
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-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
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 Clostridium botulinum
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 163-167 U/ml by MBA assay, 153 U/ml by RSC assay Clostridium botulinum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information standard assay used to determine potency of clinical samples is the in vivo mouse bioassay, MBA, another possibilty is the primary rat spinal cord cells using RSC assay, that also permits sensitive and quantitative detection of BoNT/A, with usage of Sprague Dawley E15 rat pup spinal cords, Direct comparison of MBA and RSC assays, overview Clostridium botulinum ?
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 ?
SNAP-25 + H2O i.e. 25-kDa synaptosomal-associated protein Clostridium botulinum ?
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 ?

Synonyms

Synonyms Comment Organism
BoNT/A
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 Clostridium botulinum
botulinum neurotoxin type A
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 Clostridium botulinum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Clostridium botulinum