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Literature summary for 3.4.24.69 extracted from

  • Dong, J.; Thompson, A.A.; Fan, Y.; Lou, J.; Conrad, F.; Ho, M.; Pires-Alves, M.; Wilson, B.A.; Stevens, R.C.; Marks, J.D.
    A single-domain llama antibody potently inhibits the enzymatic activity of botulinum neurotoxin by binding to the non-catalytic alpha-exosite binding region (2010), J. Mol. Biol., 397, 1106-1118.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
drug development the BoNT/A Lc alpha-exosite is a target for inhibitor development Clostridium botulinum

Inhibitors

Inhibitors Comment Organism Structure
additional information construction of a non-immune llama single-domain library for display on the surface of Saccharomyces cerevisiae and identification of a single-domain llama antibody that potently inhibits the enzymatic activity of BoNT/A light chain by binding to the non-catalytic alpha-exosite binding region, overview Clostridium botulinum
single-domain llama antibody Aa1 most potent antibody isolated from a single domain VHH, i.e. camelid heavy-chain variable region derived from heavy-chain-only antibody, antibodies, it is resistant to heat denaturation and reducing conditions. The Aa1 paratope coincides with an alpha-helical portion of the SNAP25 substrate. Structure of BoNT/A Lc-Aa1 VHH complex and inhibition mechanism, overview Clostridium botulinum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
SNAP-25 + H2O Clostridium botulinum i.e. 25-kDa synaptosome-associated protein, a substrate of BoNT/A light chain ?
-
?

Organism

Organism UniProt Comment Textmining
Clostridium botulinum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
SNAP-25 + H2O i.e. 25-kDa synaptosome-associated protein, a substrate of BoNT/A light chain Clostridium botulinum ?
-
?

Synonyms

Synonyms Comment Organism
BoNT/A
-
Clostridium botulinum
BoNT/A Lc endopeptidase
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Clostridium botulinum
botulinum neurotoxin serotype BA
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Clostridium botulinum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00000047
-
pH not specified in the publication, temperature not specified in the publication Clostridium botulinum single-domain llama antibody Aa1