Application | Comment | Organism |
---|---|---|
drug development | the BoNT/A Lc alpha-exosite is a target for inhibitor development | Clostridium botulinum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | construction of a non-immune llama single-domain library for display on the surface of Saccharomyces cerevisiae and identification of a single-domain llama antibody that potently inhibits the enzymatic activity of BoNT/A light chain by binding to the non-catalytic alpha-exosite binding region, overview | Clostridium botulinum | |
single-domain llama antibody Aa1 | most potent antibody isolated from a single domain VHH, i.e. camelid heavy-chain variable region derived from heavy-chain-only antibody, antibodies, it is resistant to heat denaturation and reducing conditions. The Aa1 paratope coincides with an alpha-helical portion of the SNAP25 substrate. Structure of BoNT/A Lc-Aa1 VHH complex and inhibition mechanism, overview | Clostridium botulinum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
SNAP-25 + H2O | Clostridium botulinum | i.e. 25-kDa synaptosome-associated protein, a substrate of BoNT/A light chain | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium botulinum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
SNAP-25 + H2O | i.e. 25-kDa synaptosome-associated protein, a substrate of BoNT/A light chain | Clostridium botulinum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BoNT/A | - |
Clostridium botulinum |
BoNT/A Lc endopeptidase | - |
Clostridium botulinum |
botulinum neurotoxin serotype BA | - |
Clostridium botulinum |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00000047 | - |
pH not specified in the publication, temperature not specified in the publication | Clostridium botulinum | single-domain llama antibody Aa1 |