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Literature summary for 3.4.24.69 extracted from

  • Shi, X.; Curran, J.E.; Liao, Z.; Gordon, R.K.
    The biological activity of ubiquitinated BoNT/B light chain in vitro and in human SHSY-5Y neuronal cells (2009), J. Cell. Biochem., 108, 660-667.
    View publication on PubMed
Search for more literature for 3.4.24.69

Application

Application Comment Organism
drug development the toxin is a target for development of inhibitors to treat muscular paralysis, and of drugs altering BoNT/B light chain ubiquitination to overcome botulinum persistence in neuronal cells Clostridium botulinum

Inhibitors

Inhibitors Comment Organism Structure
expoxomicin increases ubiquitination of BoNT/B light chain in neuronal cells. Ubiquitination in vitro and in cells decreases the biological activity of BoNT/B light chain Clostridium botulinum
additional information BoNT/B LC is processed for removal via the proteasome-dependent degradation pathway after ubiquitination in neuronal cells Clostridium botulinum
phorbol 12-myristate 13-acetate increases ubiquitination of BoNT/B light chain in neuronal cells. Ubiquitination in vitro and in cells decreases the biological activity of BoNT/B light chain Clostridium botulinum

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ a zinc-dependent endopeptidase Clostridium botulinum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
VAMP 2 + H2O Clostridium botulinum i.e. synaptobrevin-2 or vesicle-associated membrane protein 2 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Clostridium botulinum
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information BoNT/B LC is processed for removal via the proteasome-dependent degradation pathway after ubiquitination in neuronal cells Clostridium botulinum

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information assay method measuring noradrenaline release in human neuronal SHSY-5Y cells Clostridium botulinum ?
-
 ?
VAMP 2 + H2O i.e. synaptobrevin-2 or vesicle-associated membrane protein 2 Clostridium botulinum ?
-
 ?
VAMP 2 + H2O i.e. synaptobrevin-2 or vesicle-associated membrane protein 2, with BoNT/B light chain Clostridium botulinum ?
-
 ?
VAMPTide + H2O a VAMP-2-derived peptide substrate, modified with FRET, with BoNT/B light chain Clostridium botulinum ?
-
 ?

Synonyms

Synonyms Comment Organism
BoNT/B
-
 Clostridium botulinum
botulinum neurotoxin B
-
 Clostridium botulinum

General Information

General Information Comment Organism
additional information the toxin persists in neuronal cells for an extended period, it maintaines its ability to cleave VAMP2 in human neuronal cells for weeks Clostridium botulinum
physiological function after entering its target, the neuronal cell, BoNT/B is responsible for synaptobrevin-2, i.e. VAMP2, cleavage. This results in reduced neurotransmitter acetylcholine release from synaptic vesicles, yielding muscular paralysis Clostridium botulinum