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Literature summary for 3.4.24.69 extracted from
- Cloning and expression of a region of vesicle associated membrane protein2 (VAMP2) gene and its use as a recombinant peptide substrate for assaying clostridial neurotoxins in contaminated biologicals (2010), Biologicals, 38, 113-119.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | the recombinant VAMP2 peptide substrate can be used as in vitro bioassay for the detection of BONT/B in food samples and provide a relevant replacement assay for the estimation of the toxin potency in contaminated therapeutic preparations | Clostridium botulinum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| VAMP2 + H2O | Clostridium botulinum | i.e. intracellular vesicle associated membrane protein 2 | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium botulinum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native BONT/B from Clostridium botulinum by ion exchange chromatography | Clostridium botulinum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| VAMP2 + H2O | i.e. intracellular vesicle associated membrane protein 2 | Clostridium botulinum | ? | - |
? | |
| VAMP2 peptide + H2O | a synthetic peptide substrate representing amino acid residues 60-94 of the intracellular vesicle associated membrane protein 2, i.e. VAMP2, recombinant GST fusion protein and commercial preparation as substrates with equal activity for BONT/B | Clostridium botulinum | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BoNT/B | - |
Clostridium botulinum |
| botulinum neurotoxin type B | - |
Clostridium botulinum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Clostridium botulinum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Clostridium botulinum |
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