Protein Variants | Comment | Organism |
---|---|---|
additional information | single point mutations in VAMP-2 for analysation of binding kinetics to TeNT | Clostridium tetani |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | mutations at residues 41-51 of substrate vesicle-associated membrane protein 2 show dramatic effects on LC/TeNT cleavage with 320-fold inhibition by the individual mutations, E41A, V42A, V43A, and R47A. Deletion of the N-terminal 50 residues of vesicle-associated membrane protein 2 causes a 500-fold reduction in LC/TeNT efficiency. Mutation D44A shows a 10-fold higher Km (46 microM) and 10-fold lower Turnover Number (0.018 1/sec) than wild-type vesicle-associated membrane protein 2 | Clostridium tetani |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium tetani | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | mapping experiment shows that residues 40-87 of vesicle-associated membrane protein 2 are sufficient for efficient TeNT cleavage. Mutations in vesicle-associated membrane protein 2 for analysation of binding kinetics to TeNT | Clostridium tetani | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TeNT | - |
Clostridium tetani |
Tetanus neurotoxin | - |
Clostridium tetani |