Application | Comment | Organism |
---|---|---|
drug development | botulinum toxins are targets for rational development of preventive vaccines or inhibitors | Clostridium botulinum |
additional information | botulinum neurotoxins are biological hazards to humans and a serious potential bioweapon threat | Clostridium botulinum |
Cloned (Comment) | Organism |
---|---|
expression of Strep-tagged HCB-Syt-II fusion protein | Clostridium botulinum |
Crystallization (Comment) | Organism |
---|---|
purified recombinant protein consisting of the receptor-binding domain of botulinum neurotoxin serotype B fused to the luminal domain of synaptotagmin II, vapour diffusion method, 20°C, using first 13% PEG 6000 and 0.1 M HEPES pH 7.0, and second, 0.8 M sodium citrate, pH 6.5, X-ray diffraction structure detremination and analysis at 2.15 A resolution, molecular replacement and modeling | Clostridium botulinum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a HCB-Syt-II fusion protein by fusion of the HC fragment of BoNT/B, residues 858-1291, with the luminal domain of rat Syt-II, residues 861, connected by a 15-residue linker, PPTPGSAWSHPQFEK, including a Strep-tag, site-directed mutagenesis analysis of the toxin-receptor binding site in BoNT/B and in the luminal domains of Syt-I and Syt-II, overview | Clostridium botulinum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Clostridium botulinum | BoNTs bind with high specificity at neuromuscular junctions and they impair exocytosis of synaptic vesicles containing acetylcholine through specific proteolysis of SNAREs, soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptors, which constitute part of the synaptic vesicle fusion machinery, botulinum neurotoxins cause the neuroparalytic syndrome of botulism, BoNTs are biological hazard to humans and a serious potential bioweapon threat with a lethal dose of 1 ng/kg body weight | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium botulinum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | BoNTs bind with high specificity at neuromuscular junctions and they impair exocytosis of synaptic vesicles containing acetylcholine through specific proteolysis of SNAREs, soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptors, which constitute part of the synaptic vesicle fusion machinery, botulinum neurotoxins cause the neuroparalytic syndrome of botulism, BoNTs are biological hazard to humans and a serious potential bioweapon threat with a lethal dose of 1 ng/kg body weight | Clostridium botulinum | ? | - |
? | |
additional information | the the receptor-binding domain of botulinum neurotoxin serotype B binds to the luminal domain of synaptotagmin II, i.e. Syt-II, interaction occurs at both neutral and acidic endosomal pH, residues Glu44 to Lys60 become structured with residues Phe47 to Ile58 forming an alpha-helix, the HCB-Syt-II complex is stabilized by extensive intermolecular interactions involving two pronounced pockets on the HCB surface, structure, overview, high selectivity of BoNT/B among synaptotagmin I and II isoforms | Clostridium botulinum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BoNT/B | - |
Clostridium botulinum |
botulinum neurotoxin B | - |
Clostridium botulinum |
botulinum neurotoxin serotype B | - |
Clostridium botulinum |
HCB | - |
Clostridium botulinum |