Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.69 extracted from

  • Agarwal, R.; Binz, T.; Swaminathan, S.
    Analysis of active site residues of botulinum neurotoxin E by mutational, functional, and structural studies: Glu335Gln is an apoenzyme (2005), Biochemistry, 44, 8291-8302.
    View publication on PubMed
Search for more literature for 3.4.24.69

Crystallization (Commentary)

Crystallization (Comment) Organism
botulinum neurotoxin E light chain, sitting drop vapor diffusion method Clostridium botulinum

Protein Variants

Protein Variants Comment Organism
E158A/T159A/N160A kcat/KM for synaptosome-associated protein SNAP-25 is 7.4fold lower than the wild-type value Clostridium botulinum
E212A/E335Q no detectable activity with synaptosome-associated protein SNAP-25 Clostridium botulinum
E249A kcat/KM for synaptosome-associated protein SNAP-25 is 20fold lower than the wild-type value Clostridium botulinum
E335A kcat/KM for synaptosome-associated protein SNAP-25 is 37fold lower than the wild-type value Clostridium botulinum
E335Q kcat/KM for synaptosome-associated protein SNAP-25 is 7300fold lower than the wild-type value. Mutation causes the toxin to transform into a persistent apoenzyme decoid of zinc Clostridium botulinum
R347A kcat/KM for synaptosome-associated protein SNAP-25 is 1060fold lower than the wild-type value Clostridium botulinum
Y350A no detectable activity with synaptosome-associated protein SNAP-25 Clostridium botulinum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0079
-
 synaptosome-associated protein SNAP-25 37°C, pH 7.4, wild-type enzyme Clostridium botulinum
0.0097
-
 synaptosome-associated protein SNAP-25 37°C, pH 7.4, mutant enzyme E335A Clostridium botulinum
0.01
-
 synaptosome-associated protein SNAP-25 37°C, pH 7.4, mutant enzyme E335Q Clostridium botulinum
0.0112
-
 synaptosome-associated protein SNAP-25 37°C, pH 7.4, mutant enzyme E249A Clostridium botulinum
0.0112
-
 synaptosome-associated protein SNAP-25 37°C, pH 7.4, mutant enzyme R347A Clostridium botulinum
0.0113
-
 synaptosome-associated protein SNAP-25 37°C, pH 7.4, mutant enzyme E158A/T159A/N160A Clostridium botulinum

Organism

Organism UniProt Comment Textmining
Clostridium botulinum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
botulinum neurotoxin E light chain Clostridium botulinum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
synaptosome-associated protein SNAP-25 + H2O
-
 Clostridium botulinum hydrolyzed synaptosome-associated protein SNAP-25
-
 ?

Synonyms

Synonyms Comment Organism
botulinum neurotoxin E
-
 Clostridium botulinum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00075
-
 synaptosome-associated protein SNAP-25 37°C, pH 7.4, mutant enzyme E335Q Clostridium botulinum
0.0058
-
 synaptosome-associated protein SNAP-25 37°C, pH 7.4, mutant enzyme R347A Clostridium botulinum
0.144
-
 synaptosome-associated protein SNAP-25 37°C, pH 7.4, mutant enzyme E335A Clostridium botulinum
0.4
-
 synaptosome-associated protein SNAP-25 37°C, pH 7.4, mutant enzyme E249A Clostridium botulinum
0.836
-
 synaptosome-associated protein SNAP-25 37°C, pH 7.4, mutant enzyme E158A/T159A/N160A Clostridium botulinum
3.13
-
 synaptosome-associated protein SNAP-25 37°C, pH 7.4, wild-type enzyme Clostridium botulinum
4.29
-
 synaptosome-associated protein SNAP-25 37°C, pH 7.4, wild-type enzyme Clostridium botulinum
6.08
-
 synaptosome-associated protein SNAP-25 37°C, pH 7.4, mutant enzyme E158A/T159A/N160A Clostridium botulinum