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Literature summary for 3.4.24.69 extracted from

  • Schmidt, J.J.; Bostian, K.A.
    Endoproteinase activity of type A botulinum neurotoxin: substrate requirements and activation by serum albumin (1997), J. Protein Chem., 16, 19-26.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin stimulates hydrolysis of synthetic peptides Clostridium botulinum
goat serum albumin stimulates hydrolysis of synthetic peptides Clostridium botulinum
horse serum albumin stimulates hydrolysis of synthetic peptides Clostridium botulinum
Human serum albumin stimulates hydrolysis of synthetic peptides Clostridium botulinum
additional information stimulation specific to serum albumins, bovine gamma globulin, ovalbumin, lysozyme, ribonuclease, and gelatin have no effect Clostridium botulinum
rabbit serum albumin stimulates hydrolysis of synthetic peptides Clostridium botulinum
sheep serum albumin stimulates hydrolysis of synthetic peptides Clostridium botulinum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.58
-
SNKTRIDEANQRATKXL pH 7.3, 37°C Clostridium botulinum
0.6
-
SNKTRIDEAAQRATKML pH 7.3, 37°C Clostridium botulinum
0.75
-
SNKTRIDQANQRATKML pH 7.3, 37°C Clostridium botulinum
0.75
-
SNKTRIDEANNRATKML pH 7.3, 37°C Clostridium botulinum
0.82
-
SNKTRINEANQRATKML pH 7.3, 37°C Clostridium botulinum
0.83
-
SNKTRIDEANBRATKML pH 7.3, 37°C Clostridium botulinum
0.9
-
SNKTRIDEANQRATK pH 7.3, 37°C Clostridium botulinum
1
-
SNKTRIDEANQRATAML pH 7.3, 37°C Clostridium botulinum
1.1
-
SNKTRIDEBNQRATKML pH 7.3, 37°C Clostridium botulinum
1.2
-
SNKTRIDEANQRATKML pH 7.3, 37°C Clostridium botulinum
1.3
-
SNKTRIDEANQRABKML pH 7.3, 37°C Clostridium botulinum
1.6
-
SNKTRIDEANQRATKM pH 7.3, 37°C Clostridium botulinum
1.7
-
SNKTRIDEANQRATKML pH 7.3, 37°C Clostridium botulinum
1.8
-
SNKTRIDEANQRBTKML pH 7.3, 37°C Clostridium botulinum
1.9
-
SNKTRIDEANQRATKAL pH 7.3, 37°C Clostridium botulinum

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ zinc-dependent endoproteinase Clostridium botulinum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Clostridium botulinum most potent toxin known ?
-
?

Organism

Organism UniProt Comment Textmining
Clostridium botulinum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information most potent toxin known Clostridium botulinum ?
-
?
neuronal proteinSNAP-25 + H2O
-
Clostridium botulinum ?
-
?
SNKTRIDEAAQRATKML + H2O synthetic peptide substrate Clostridium botulinum SNKTRIDEAAQ + RATKML
-
?
SNKTRIDEANBRATKML + H2O synthetic peptide substrate Clostridium botulinum SNKTRIDEANB + RATKML
-
?
SNKTRIDEANNRATKML + H2O synthetic peptide substrate Clostridium botulinum SNKTRIDEANN + RATKML
-
?
SNKTRIDEANQRABKML + H2O synthetic peptide substrate Clostridium botulinum SNKTRIDEANQ + RABKML
-
?
SNKTRIDEANQRASKML + H2O synthetic peptide substrate Clostridium botulinum SNKTRIDEANQ + RASKML
-
?
SNKTRIDEANQRATAML + H2O synthetic peptide substrate Clostridium botulinum SNKTRIDEANQ + RATAML
-
?
SNKTRIDEANQRATK + H2O synthetic peptide substrate Clostridium botulinum SNKTRIDEANQ + RATK
-
?
SNKTRIDEANQRATKAL + H2O synthetic peptide substrate Clostridium botulinum SNKTRIDEANQ + RATKAL
-
?
SNKTRIDEANQRATKM + H2O synthetic peptide substrate Clostridium botulinum SNKTRIDEANQ + RATKM
-
?
SNKTRIDEANQRATKML + H2O synthetic peptide substrate Clostridium botulinum SNKTRIDEANQ + RATKML
-
?
SNKTRIDEANQRATKXL + H2O synthetic peptide substrate Clostridium botulinum SNKTRIDEANQ + RATKXL
-
?
SNKTRIDEANQRBTKML + H2O synthetic peptide substrate Clostridium botulinum SNKTRIDEANQ + RBTKML
-
?
SNKTRIDEBNQRATKML + H2O synthetic peptide substrate Clostridium botulinum SNKTRIDEBNQ + RATKML
-
?
SNKTRIDQANQRATKML + H2O
-
Clostridium botulinum ?
-
?
SNKTRINEAAQRATKML + H2O synthetic peptide substrate Clostridium botulinum SNKTRINEAAQ + RATKML
-
?
SNKTRINEANQRATKML + H2O
-
Clostridium botulinum ?
-
?

Synonyms

Synonyms Comment Organism
botox A
-
Clostridium botulinum
type A botulinum neurotoxin
-
Clostridium botulinum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
SNKTRIDEANQRATK pH 7.3, 37°C Clostridium botulinum
1.8
-
SNKTRIDEAAQRATKML pH 7.3, 37°C Clostridium botulinum
5
-
SNKTRINEANQRATKML pH 7.3, 37°C Clostridium botulinum
7.7
-
SNKTRIDEANBRATKML pH 7.3, 37°C Clostridium botulinum
8
-
SNKTRIDEANQRATAML pH 7.3, 37°C Clostridium botulinum
9
-
SNKTRIDEANQRATKML pH 7.3, 37°C Clostridium botulinum
11
-
SNKTRIDEBNQRATKML pH 7.3, 37°C Clostridium botulinum
19
-
SNKTRIDEANNRATKML pH 7.3, 37°C Clostridium botulinum
25
-
SNKTRIDEANQRATKAL pH 7.3, 37°C Clostridium botulinum
30
-
SNKTRIDEANQRATKXL pH 7.3, 37°C Clostridium botulinum
35
-
SNKTRIDEANQRABKML pH 7.3, 37°C Clostridium botulinum
39
-
SNKTRIDEANQRBTKML pH 7.3, 37°C Clostridium botulinum
47
-
SNKTRIDEANQRATKML pH 7.3, 37°C Clostridium botulinum
51
-
SNKTRIDQANQRATKML pH 7.3, 37°C Clostridium botulinum
56
-
SNKTRIDEANQRATKM pH 7.3, 37°C Clostridium botulinum