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Literature summary for 3.4.24.69 extracted from

  • Li, L.; Singh, B.R.
    Role of zinc binding in type A botulinum neurotoxin light chain's toxic structure (2000), Biochemistry, 39, 10581-10586.
    View publication on PubMed
Search for more literature for 3.4.24.69

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.041
-
 SNAP-25 pH 7.0, 37°C, holo-light chain Clostridium botulinum
0.055
-
 SNAP-25 pH 7.0, 37°C, light chain Zn2+ replenished Clostridium botulinum

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ zinc endopeptidase, contains 1 Zn2+/molecule Clostridium botulinum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 light chain, enzymatic subunit Clostridium botulinum

Organism

Organism UniProt Comment Textmining
Clostridium botulinum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
SNAP-25 + H2O synaptosomal-associated protein Clostridium botulinum ?
-
 ?
synaptobrevin + H2O VAMP Clostridium botulinum ?
-
 ?

Synonyms

Synonyms Comment Organism
BoNT/A
-
 Clostridium botulinum
type A botulinum neurotoxin light chain
-
 Clostridium botulinum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
44 52 temperature denaturation Tm is significantly decreased upon removal of Zn2+ Clostridium botulinum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2
-
 SNAP-25 pH 7.0, 37°C, light chain Zn2+ replenished Clostridium botulinum
2.3
-
 SNAP-25 pH 7.0, 37°C, holo-light chain Clostridium botulinum