Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Clostridium botulinum | |
captopril | - |
Clostridium botulinum | |
EDTA | - |
Clostridium botulinum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | activation requires reduction of interchain disulfide bond | Clostridium botulinum | |
Zinc | contains zinc binding motif of metalloendopeptidases His-Glu-X-X-His | Clostridium botulinum | |
Zinc | zinc-dependent endopeptidase | Clostridium botulinum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium botulinum | - |
serotypes BoNT/A, BoNT/B | - |
Clostridium botulinum | - |
BoNT/C, BoNT/D | - |
Clostridium botulinum | - |
type G strain | - |
Clostridium botulinum type G | - |
type G strain | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | catalytic activity requires reduction of the single interchain disulfide bond of the neurotoxin | Clostridium botulinum | ? | - |
? | |
additional information | the clostridial neurotoxins differ from other proteases in the recognition of the tertiary structure of the target rather than the sequence of the peptide bond to be cleaved | Clostridium botulinum | ? | - |
? | |
additional information | synaptotagmin, synaptophysin | Clostridium botulinum | ? | - |
? | |
additional information | catalytic activity requires reduction of the single interchain disulfide bond of the neurotoxin | Clostridium botulinum type G | ? | - |
? | |
additional information | the clostridial neurotoxins differ from other proteases in the recognition of the tertiary structure of the target rather than the sequence of the peptide bond to be cleaved | Clostridium botulinum type G | ? | - |
? | |
additional information | synaptotagmin, synaptophysin | Clostridium botulinum type G | ? | - |
? | |
Recombinant glutathione S-methyltransferase VAMP-2 fusion protein + H2O | - |
Clostridium botulinum | Hydrolyzed recombinant glutathione S-methyltransferase VAMP-2 fusion protein | 2 proteolytic fragments, MW 36000 and MW 6000 | ? | |
Synaptobrevin + H2O | i.e. VAMP, neuronal vesicle-associated membrane protein, MW 19000, with 2 isoforms in human, chicken, in rat brain: synaptobrevin/VAMP-1 and synaptobrevin/VAMP-2, cleaves at Gln76-Phe77, the same site as botulin neurotoxin B | Clostridium botulinum | Hydrolyzed synaptobrevin | - |
? | |
Synaptobrevin + H2O | serotype BoNT/B: cleavage at -Asp-Gln-+-Lys-Leu-, serotype BoNT/G: cleavage at Ala83-Ala84 (VAMP-1), Ala81-Ala82 (VAMP-2) | Clostridium botulinum | Hydrolyzed synaptobrevin | - |
? | |
Synaptobrevin + H2O | carrying synaptobrevin/VAMP-2 | Clostridium botulinum | Hydrolyzed synaptobrevin | - |
? | |
Synaptobrevin + H2O | both isoforms are cleaved at the same rate | Clostridium botulinum | Hydrolyzed synaptobrevin | - |
? | |
Synaptobrevin + H2O | carrying Val76 instead of Gln76 is not hydrolyzed by serotype BoNT/B | Clostridium botulinum | Hydrolyzed synaptobrevin | - |
? | |
Synaptobrevin + H2O | highly specific neurotoxins | Clostridium botulinum | Hydrolyzed synaptobrevin | - |
? | |
Synaptobrevin + H2O | i.e. VAMP, neuronal vesicle-associated membrane protein, MW 19000, with 2 isoforms in human, chicken, in rat brain: synaptobrevin/VAMP-1 and synaptobrevin/VAMP-2, cleaves at Gln76-Phe77, the same site as botulin neurotoxin B | Clostridium botulinum type G | Hydrolyzed synaptobrevin | - |
? | |
Synaptobrevin + H2O | serotype BoNT/B: cleavage at -Asp-Gln-+-Lys-Leu-, serotype BoNT/G: cleavage at Ala83-Ala84 (VAMP-1), Ala81-Ala82 (VAMP-2) | Clostridium botulinum type G | Hydrolyzed synaptobrevin | - |
? | |
Synaptosome-associated protein + H2O | no substrate of serotype BoNT/G | Clostridium botulinum | Hydrolyzed synaptosome-associated protein | - |
? | |
Syntaxin + H2O | no substrate of serotype BoNT/G | Clostridium botulinum | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Clostridium botulinum |